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| Product Name | SDS-PAGE Gel Preparation Kit (Tris-Glycine System) |
| Catalog No. | PTR-HMM-0060 |
| Description | A complete reagent kit for the preparation of polyacrylamide gels for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The kit includes all required buffers and reagents for resolving and stacking gel preparation using the standard Laemmli system. |
| Intended Use | Preparation of denaturing polyacrylamide gels for protein separation by molecular weight in SDS-PAGE, a fundamental technique in protein biochemistry and molecular biology. |
| Principle / Technology | Proteins are denatured by SDS and migrate through the polyacrylamide gel matrix under an electric field at rates inversely proportional to the logarithm of their molecular weight. |
| Detection Method | Electrophoretic separation; subsequent visualization by Coomassie staining, silver staining, or Western blot transfer |
| Sample Type | Denatured protein samples (1–100 μg per lane) in loading buffer containing SDS and reducing agent |
| Performance Range / Specifications | Resolution range: 10–250 kDa (8% gel), 6–80 kDa (12% gel), 2–40 kDa (15% gel); adjustable acrylamide percentage for optimal resolution |
| Sensitivity / LOD | Detection limit: ~10 ng per band (Coomassie stain), ~0.1 ng per band (silver stain) — dependent on staining method |
| Specificity | Separation based on molecular weight; does not distinguish between protein isoforms with similar molecular weights |
| Reaction Conditions / Protocol | Prepare resolving gel solution, pour between glass plates, overlay with water; after polymerization, prepare stacking gel solution, pour, insert comb; gels ready in approximately 1 hour |
| Components / Formulation | 30% Acrylamide/Bis-acrylamide solution (29:1 ratio), 1.5 M Tris-HCl pH 8.8 (resolving gel buffer), 0.5 M Tris-HCl pH 6.8 (stacking gel buffer), 10% SDS solution, 10% ammonium persulfate (APS), TEMED, detailed protocol |
| Storage Conditions | 2–8°C; APS solution should be prepared fresh weekly; TEMED stored at room temperature in dark; acrylamide solution is light-sensitive |
| Shelf Life | 12 months (acrylamide solution); 24 months (buffer components) |
| Package Specifications | Sufficient for 40–60 mini-gels (8 x 10 cm) |
| Product Form | Liquid reagents with APS as crystalline powder (reconstitute before use) |
| Quality Control | Acrylamide solution tested for polymerization efficiency; each lot validated by SDS-PAGE with molecular weight markers; endotoxin tested for cell culture-related applications |
| Key Features | Adjustable gel percentage for customized protein resolution; pre-mixed acrylamide/bis-acrylamide eliminates weighing steps; all buffers pH-adjusted and ready to use |
For research use only, not for clinical use.
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