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| Product Name | DTT (Dithiothreitol) Reducing Agent, Molecular Biology Grade |
| Catalog No. | PTR-HMM-0076 |
| Description | An ultra-pure preparation of dithiothreitol for the reduction of disulfide bonds in proteins and peptides, used in SDS-PAGE sample preparation, protein refolding, and enzymatic reaction buffers. |
| Intended Use | Reduction of disulfide bonds in proteins for SDS-PAGE denaturation, maintenance of reduced cysteine thiols in enzymatic assays, and prevention of protein oxidation during purification. |
| Principle / Technology | DTT reduces disulfide bonds through thiol-disulfide exchange, with the formation of a stable six-membered ring intramolecular disulfide in the oxidized DTT product driving the equilibrium toward complete reduction. |
| Detection Method | Proteins treated with DTT are analyzed by SDS-PAGE, mass spectrometry, or enzymatic activity assays |
| Sample Type | Purified proteins, cell lysates, enzyme preparations, and peptide samples requiring disulfide reduction |
| Performance Range / Specifications | Effective reducing concentration: 1–100 mM depending on application; typical SDS-PAGE loading buffer concentration: 50–100 mM |
| Sensitivity / LOD | Effective reduction of protein disulfide bonds at concentrations as low as 1 mM under optimal conditions |
| Specificity | Reduces accessible disulfide bonds in all proteins; does not reduce non-disulfide covalent modifications |
| Reaction Conditions / Protocol | Dissolve DTT in ultrapure water or appropriate buffer at the desired concentration immediately before use; add to protein samples and incubate at 95°C for 5 minutes (SDS-PAGE) or at room temperature for 30 minutes (native reduction) |
| Components / Formulation | DTT crystalline powder, pre-weighed in 5 g container; solubility >50 mg/mL in water |
| Storage Conditions | -20°C desiccated and protected from moisture; DTT is hygroscopic and air-sensitive; working solutions should be prepared fresh |
| Shelf Life | 36 months from date of manufacture |
| Package Specifications | 5 g |
| Product Form | White crystalline powder |
| Quality Control | Purity >99% by HPLC; heavy metal content <5 ppm; DNase, RNase, and protease tested; functional activity verified by reduction of a model disulfide-containing protein |
| Key Features | Ultra-pure grade suitable for the most demanding molecular biology and biochemical applications; pre-weighed format reduces handling of hygroscopic powder; the intramolecular disulfide formation in oxidized DTT drives complete reduction |
For research use only, not for clinical use.
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