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| Product Name | GST-Tag Protein Purification Resin (Glutathione Agarose) |
| Catalog No. | PTR-HMM-0070 |
| Description | A glutathione-coupled agarose resin for the affinity purification of recombinant proteins fused to glutathione S-transferase. The resin provides high-capacity capture of GST-tagged proteins from bacterial, yeast, and mammalian expression systems. |
| Intended Use | Affinity purification of GST-fusion proteins from clarified cell lysates under native conditions for biochemical characterization, antibody production, and functional studies. |
| Principle / Technology | Immobilized glutathione on cross-linked agarose beads binds specifically to the glutathione S-transferase affinity tag with high selectivity; bound GST-fusion proteins are eluted with reduced glutathione under mild, non-denaturing conditions. |
| Detection Method | Column or batch-mode chromatography with UV detection at 280 nm |
| Sample Type | Clarified lysates from cells expressing GST-fusion recombinant proteins; compatible with E. coli, yeast, and mammalian expression systems |
| Performance Range / Specifications | Binding capacity: ≥10 mg of GST protein per milliliter of settled resin; typical elution purity exceeds 90% in a single step |
| Sensitivity / LOD | Sufficient for purifying GST-fusion proteins from low-expression constructs with optimization of binding conditions |
| Specificity | High selectivity for GST and GST-fusion proteins; minimal non-specific binding of host cell proteins under recommended buffer conditions |
| Reaction Conditions / Protocol | Equilibrate column with PBS, load clarified cell lysate at a flow rate of 0.5–1 mL/min, wash column with 10–20 column volumes of PBS, elute with 50 mM Tris-HCl pH 8.0 containing 10 mM reduced glutathione, collect fractions and analyze by SDS-PAGE |
| Components / Formulation | Glutathione Sepharose resin as a 50% slurry in 20% ethanol, included: 10× PBS concentrate, reduced glutathione elution buffer powder, empty chromatography columns |
| Storage Conditions | 2–8°C; do not freeze the resin; storage solution contains 20% ethanol as preservative |
| Shelf Life | 24 months |
| Package Specifications | 10 mL of 50% slurry (5 mL bed volume) |
| Product Form | 50% bead slurry in ethanol-containing storage buffer |
| Quality Control | Binding capacity validated using purified recombinant GST protein; resin chemical stability tested; glutathione leaching measured by HPLC |
| Key Features | Mild elution conditions preserve native protein conformation and enzymatic activity; high selectivity reduces purification steps; resin is compatible with standard laboratory chromatography equipment |
For research use only, not for clinical use.
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