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| Product Name | Albumin Content Assay Reagent-Bromocresol Purple Colorimetry (Microplate Reader) |
| Catalog No. | PTR-HMM-0052 |
| Description | Albumin is an important plasma protein primarily synthesized and secreted by the liver into the bloodstream. It performs multiple vital functions and physiological roles, including binding with various nutrients, hormones, and medications to maintain nutritional balance and plasma osmotic pressure. Additionally, it plays a significant role in immune regulation and antioxidant processes. |
| Application | Albumin carries a positive charge in an acidic environment. In the presence of nonionic surfactants, it can combine with negatively charged bromocresol purple (BCP) to form a green complex. The product has a characteristic absorption peak at 603 nm, and the albumin content can be quantitatively detected by measuring the change in absorbance. |
| Applicable Instruments | Microplate Reader |
| Number of Testable Samples | 96 Samples |
| Matching | 96-well plate |
| Detection Time | 4 h (96 Samples) |
| Detection Method | Bromocresol Purple Colorimetry |
| Spectral Parameters | 603 nm |
| Signal Response | Incremental |
| Notes | If the ∆A measurement is greater than 0.4, it is recommended to dilute the sample with the extraction solution before measurement; If the ∆A measurement is less than 0.01 or the A measurement is close to the A blank, it is recommended to increase the sample volume appropriately before performing the measurement again, and make corresponding adjustments during calculation. If the sample becomes turbid after adding the color developer, it is recommended to dilute the sample appropriately with the extraction solution before performing the measurement again, and make corresponding adjustments during calculation. |
For research use only, not for clinical use.
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