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| Product Name | Free Cholesterol Fluorometric Assay Kit |
| Catalog No. | BLTR-0021 |
| Description | This free cholesterol fluorometric assay kit uses Amplex Red fluorometric method for ultra - sensitive FC detection. It has excellent precision, suitable for studying free cholesterol's role in cell membrane function and lipid metabolism |
| Test Target | Free Cholesterol (FC) |
| Methodology | Fluorometric (Amplex Red, horseradish peroxidase, cholesterol oxidase) |
| Measurement Range | 0.01-5 μM |
| Sample Type | Serum, plasma, cell lysates, membrane fractions |
| Assay Time | 25 min at 37°C |
| Wavelength | 560 nm (excitation), 590 nm (emission) |
| Linearity | 0.01-5 μM |
| Precision (Intra-assay CV) | ≤1.5% |
| Precision (Inter-assay CV) | ≤2.5% |
| Stability (Unopened) | 24 months at -20°C |
| Stability (Opened) | 60 days at 2-8°C |
| Stability (Reconstituted) | 14 days at 2-8°C |
| Interference (Hemoglobin) | ≤500 mg/dL (no significant interference) |
| Interference (Bilirubin) | ≤20 mg/dL (no significant interference) |
| Interference (Lipemic) | ≤1000 mg/dL (no significant interference) |
| Reagent Composition | Amplex Red reagent, horseradish peroxidase, cholesterol oxidase, buffer |
| Reagent Form | Liquid |
| Reagent Volume | R1: 10 mL; R2: 5 mL |
| Calibration Type | External calibration |
| Calibration Levels | 5 levels |
| Incubation Temperature | 37°C |
| Detection Instrument | Fluorescence microplate reader |
| Application | High - sensitivity quantitative determination of free cholesterol in biological samples for research on cell membrane cholesterol metabolism and signal transduction |
| Cross - Reactivity | No cross - reactivity with cholesterol esters, other sterols |
| pH Range | 7.0-7.5 |
| Shelf Life | 24 months |
| Storage Condition | Store unopened at -20°C; opened and reconstituted at 2-8°C, protected from light |
For research use only, not for clinical use.
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