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| Product Name | FokI Rapid Restriction Endonuclease (5 U/uL) |
| Catalog No. | IVDRM-0419 |
| Description | Recognising 5 prime GGATG(N)9^ 3 prime, this rapid recombinant endonuclease cleaves 9 bases downstream on one strand and 13 bases on the other, outside its asymmetric recognition sequence, a distal cutting behaviour widely exploited in engineered nuclease and cloning designs. It digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 5 U/uL, and operates in the shared rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. Dam, CpG and EcoBI methylation block cleavage at overlapping sites while Dcm and EcoKI methylation are tolerated, and BstF5I, BtsCI and BseGI are documented isoschizomers. |
| Intended Use | DNA fragment preparation and genome engineering workflows leveraging distal cleavage outside the recognition site |
| Principle / Technology | Asymmetric cleavage 9 and 13 bases outside 5 prime GGATG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Dam, CpG and EcoBI methylation block cleavage; Dcm and EcoKI have no effect; isoschizomers BstF5I, BtsCI and BseGI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | Distal cleavage 9 and 13 bases from the site; single shared buffer across the rapid series |
| Concentration | 5 U/uL |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
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