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| Product Name | Filter Pipette Tips, 10 uL, Sterile, Low-Retention |
| Catalog No. | IGM-HMM-0037 |
| Description | Aerosol-barrier filter tips with integrated hydrophobic polyethylene filter element preventing aerosol and liquid contamination of the pipette shaft. Low-retention surface treatment reduces sample binding to internal surfaces. Sterilized by gamma irradiation. |
| Intended Use | Critical liquid handling for sensitive molecular biology applications including qPCR setup, NGS library preparation, single-cell workflows, and infectious sample processing where cross-contamination must be absolutely prevented. |
| Principle / Technology | Sintered polyethylene filter plug with controlled porosity (approximately 40 um pore size) acts as a physical barrier against aerosol transmission while allowing free air passage. Low-retention polymer surface modification reduces hydrophobic and electrostatic protein adsorption. |
| Detection Method | Aerosol barrier integrity test by dye aerosol challenge; low-retention validated by BSA recovery assay; sterility per USP <71> |
| Sample Type | DNA/RNA templates, PCR and qPCR reagents, NGS library preparations, viral samples, precious clinical specimens |
| Performance Range / Specifications | Volume accuracy +/- 2.0% at 10 uL, +/- 5.0% at 1 uL; BSA recovery >97% in low-retention format |
| Sensitivity / LOD | Filter blocks >99.9% of aerosol particles >0.3 um; RNase/DNase non-detectable |
| Specificity | No detectable PCR inhibition in qPCR using 10-fold serial dilutions of human genomic DNA standard |
| Reaction Conditions / Protocol | Aspirate and dispense with smooth, consistent plunger motion; the filter plug self-seals upon contact with liquid overfill. |
| Components / Formulation | Virgin polypropylene tip body, sintered ultra-high molecular weight polyethylene (UHMW-PE) filter plug, proprietary low-retention surface coating |
| Storage Conditions | 15-30 C, dry environment; rack orientation upright to maintain filter positioning |
| Shelf Life | 48 months from sterilization date |
| Package Specifications | 96 tips per hinged-rack box, 10 boxes per case; refill system 96 tips per insert, 10 inserts per case |
| Product Form | Racked tips with integrated aerosol filter, sterile, individually wrapped |
| Quality Control | Filter barrier integrity tested at 100% inspection using pressure decay method; lot release BSA recovery validation; bioburden and endotoxin testing |
| Key Features | Aerosol barrier prevents pipette contamination and sample cross-talk; low-retention for maximum nucleic acid and protein recovery; certified nuclease-free; sterile |
| Purity | Sterile (SAL 10^-6); RNase-free, DNase-free certified; pyrogen-free (<0.005 EU/uL); ATP-free |
| Concentration | N/A |
| Activity / Unit Definition | N/A |
| Molecular Weight | N/A |
| Source / Origin | Medical-grade polypropylene and UHMW-PE filter media; manufactured under ISO 13485 in ISO Class 7 cleanroom |
| pH Range / Optimal pH | Chemically resistant at pH 2-12; low-retention coating stable in aqueous and moderate organic solutions |
| Shipping Conditions | Ambient temperature; protect filter rack boxes from crushing and dropping |
| Expiration Date / Stability | 4 years from sterilization date; filter integrity guaranteed throughout shelf life when stored properly |
| Regulatory / Compliance | ISO 13485:2016; ISO 9001:2015; CE marked; compliant with ISO 8655 and ISO 17665 sterilization standards |
| Compatibility | Universal fit for 10 uL pipettors; compatible with PCR master mixes containing dyes and probes; suitable for automated liquid handling workstations with adapter plates |
| Recommended Buffer System | Compatible with PCR buffers (Tris-KCl-MgCl2 based), RT reaction buffers, NGS library preparation buffers, and enzyme dilution buffers |
| Application Notes / Precautions | Do not exceed nominal volume to prevent filter wetting; if filter becomes wet, replace tip immediately; low-retention tips recommended for viscous samples containing proteins or nucleic acids |
| Batch-to-Batch Consistency | Filter porosity controlled to +/- 5 um; low-retention coating thickness monitored by ellipsometry per batch; BSA recovery within +/- 2% across batches |
For research use only, not for clinical use.
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