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| Product Name | Fetal Bovine Serum (Premium Plus Grade) |
| Catalog No. | RESK-0231 |
| Description | Fetal Bovine Serum (Premium Plus Grade) is a high-quality serum supplement that is triple-filtered through 100 nm filters and screened for mycoplasma and viruses. After rigorous quality testing, the serum is filled on a fully automated production line under standardized manufacturing conditions to ensure consistent quality. It is rich in nutrients required for cell growth and is suitable for the culture of most routine cell lines and primary cells, and can also support the culture of certain stem cells. Highlights: Broad cell compatibility: suitable for the vast majority of routine cell lines, primary cells and some stem cells, including various cancer cells (liver, lung, colorectal, cervical, bladder cancer, etc.), myeloma cells, glioma cells, neuroma cells, hybridoma cells, fibroblasts, epithelial cells, neural cells, primary cells, and stem cells such as MSCs and PBMCs. Assured quality: 100% Uruguay-sourced serum with stringent mycoplasma testing and virus screening. Stable performance: healthy cell growth, short doubling times, and minimal lot-to-lot variation. |
| Intended Use | Used as a growth supplement in cell culture media for the in vitro culture of routine cell lines, primary cells and certain stem cells (e.g., MSCs and PBMCs). For research use. |
| Principle / Technology | Triple 100 nm filtration sterilization combined with mycoplasma and virus screening, followed by fully automated aseptic filling under standardized production conditions. |
| Performance Range / Specifications | Endotoxin: ≤5 EU/mL Hemoglobin: ≤0.02% (w/v) Mycoplasma: negative Sterilization: triple 100 nm filtration Blood origin: Uruguay |
| Reaction Conditions / Protocol | Thawing (choose one of two methods): 1) Remove the serum from -20°C storage and place it overnight in a 2-8°C refrigerator. Then transfer the serum to a 37°C water bath, swirling the bottle occasionally to mix. Do not leave the thawed serum at 37°C for an extended period. 2) Remove the serum directly from -20°C and place it in a 37°C water bath, shaking the bottle continuously to accelerate thawing and mixing. Once thawed, the serum can be allowed to return to room temperature and then aliquoted directly. |
| Components / Formulation | Sterile-filtered fetal bovine serum rich in nutrients and growth factors required for cell growth. |
| Storage Conditions | Store at -20°C to -10°C for up to 5 years. Once thawed, keep the serum at 2-8°C and use within 6 weeks. For long-term storage after thawing, aliquot under sterile conditions and refreeze; avoid repeated freeze-thaw cycles. |
| Shelf Life | 5 years when stored at -20°C to -10°C. |
| Package Specifications | 500 mL 50 mL |
| Product Form | Frozen liquid serum |
| Quality Control | Each lot is tested for endotoxin (≤5 EU/mL), hemoglobin (≤0.02%, w/v), mycoplasma (negative) and viruses (screened), and is sterilized by triple 100 nm filtration. Cell culture performance is verified by healthy growth status and short doubling times. |
| Source / Origin | 100% Uruguay-sourced fetal bovine blood. |
| Shipping Conditions | Shipped on dry ice. |
| Expiration Date / Stability | Stable for 5 years at -20°C to -10°C; after thawing, stable for up to 6 weeks at 2-8°C. |
| Compatibility | Compatible with a broad range of cell types, including human cancer cell lines (liver, lung, colorectal, cervical, bladder, kidney, esophageal, gastric, pancreatic, breast, ovarian, prostate cancers, glioma, neuroblastoma, melanoma, etc.), myeloma and hybridoma cells, fibroblasts, epithelial cells, neural cells, primary cells, mouse/rat/hamster/monkey cell lines (e.g., CHO, NIH 3T3, C2C12, Vero, MDCK, COS-7, PC-12), and stem cells such as MSCs and PBMCs. |
| Application Notes / Precautions | Fine sand-like or flocculent precipitates may appear after thawing; these are mainly calcium phosphate and denatured lipoproteins/fibrin and do not affect serum quality. If needed, remove them by centrifugation at 400-600 g (e.g., 500 g) for 5-10 min and filter the supernatant together with the basal medium; do not filter the precipitate directly, as it may clog the membrane. If the bottle is not shaken during thawing and the temperature exceeds 40°C, material at the bottom of the bottle may undergo protein denaturation and form precipitates. Avoid prolonged exposure of the serum to high temperatures and repeated freeze-thaw cycles. |
| Batch-to-Batch Consistency | Standardized, fully automated production delivers stable cell culture performance with short doubling times and minimal lot-to-lot variation. |
| Heat Inactivation | Heat inactivation is not required for most cell types and is not recommended for routine cell culture, as it provides little or no benefit, may reduce serum quality, and markedly increases precipitate formation. If heat inactivation is necessary, treat the serum in a 56°C water bath for 30 min, swirling several times during the process. |
For research use only, not for clinical use.
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