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| Product Name | Fetal Bovine Serum (Premium Grade) |
| Catalog No. | RESK-0227 |
| Description | Fetal Bovine Serum (Premium Grade) undergoes triple 100 nm filtration and is screened for mycoplasma and viruses. After rigorous quality testing, it is aseptically filled through a fully automated process under standardized production, ensuring guaranteed quality. It is rich in nutrients essential for cell growth and is suitable for culturing the majority of common cell lines. |
| Intended Use | Suitable for the culture of the vast majority of common cell types, including routine tumor cells, large-scale production cells (293T, CHO, VERO, etc.), and immune cells (RAW264.7, THP-1, etc.). For delicate or difficult-to-culture cells (e.g., stem cells such as NSC and PB-MSCs; difficult primary cells such as epithelial cells, endothelial cells, and cardiomyocytes; and certain breast, colorectal, lung adenocarcinoma, and glioma cells), a higher-grade serum is recommended. |
| Principle / Technology | Triple 100 nm sterile filtration with automated aseptic filling; screened for mycoplasma and viruses. |
| Sample Type | Cell culture supplement for mammalian cell culture. |
| Performance Range / Specifications | Endotoxin level: ≤ 5 EU/mL Hemoglobin content: ≤ 0.02% (w/v) Mycoplasma: negative Sterilization: triple 100 nm filtration |
| Reaction Conditions / Protocol | Thawing (choose one of the two methods): 1) Remove the serum from -20 °C storage and place it in a 2–8 °C refrigerator overnight to partially thaw, then allow it to fully thaw at room temperature, swirling the bottle occasionally to mix the contents. This method is recommended. 2) Remove the serum directly from -20 °C and place it in a 37 °C water bath, continuously swirling the bottle to accelerate thawing and mixing. Do not leave the serum in the 37 °C water bath for an extended period after thawing. Note: If the bottle is not swirled, material deposited at the bottom may undergo protein denaturation and form precipitates when the temperature exceeds 40 °C. |
| Components / Formulation | Pure natural product containing no artificially added ingredients; rich in multiple growth factors supporting stable cell culture performance. |
| Storage Conditions | Store at -20 °C to -10 °C. Once thawed, keep the serum at 2–8 °C and use within 6 weeks. For long-term storage, aliquot the serum aseptically and refreeze; avoid repeated freeze-thaw cycles. During use, avoid repeated freeze-thaw cycles and exposure to ultraviolet light. |
| Shelf Life | 5 years when stored at -20 °C to -10 °C. |
| Package Specifications | 500 mL 50 mL |
| Product Form | Frozen liquid serum |
| Quality Control | Triple 100 nm (0.1 μm) sterile filtration effectively removes microorganisms; screened for mycoplasma and viruses; endotoxin ≤ 5 EU/mL; hemoglobin ≤ 0.02% (w/v); rigorous batch testing with fully automated aseptic filling under standardized production. |
| Source / Origin | Bovine fetus (fetal bovine blood); pure natural product without any artificially added ingredients. |
| Shipping Conditions | Shipped on dry ice. |
| Expiration Date / Stability | Stable for 5 years at -20 °C to -10 °C; once thawed, store at 2–8 °C for no more than 6 weeks. |
| Compatibility | Suitable for a wide range of common cell lines, including CCRF-CEM, A-431, SP2/0, CFPAC-1, HeLa, Huh-7, PC-3, Jurkat E6-1, MRC-5, MOLM-13, MSC, RH-35, BT-474, PANC-1, SH-SY5Y, 786-O, MG-63, bEnd.3, HCT-8, Hep3B2-1-7, K-562, WI-38, NRK, PC-12, KG-1, ARPE-19, NK92, ZR-75-30, SK-MEL-5, MCF7, MDA-MB-436, MKN74, NCI-H1437, NCI-H524, NCI-H23, NCI-H661, LM3, NCI-H508, PA-1, RG2, MHCC-97H, MB49, ID8, HC11, MC38, AML12, hccc-9810, Hep G2, Calu-6, MM.1S, P19, PC-9, NCI-H226, NCI-H929, and many other human, mouse, and rat cell lines (a total of 75+ validated cell lines). |
| Application Notes / Precautions | The product has been heat-inactivated before leaving the factory; no further inactivation is generally required. Flocculent precipitates may appear after thawing, mainly due to lipoprotein denaturation and fibrin; they do not affect serum quality. To remove them, aliquot the serum into sterile centrifuge tubes and centrifuge at 400 g for 10–15 min. Filtration is not recommended as precipitates may clog the filter membrane. Follow proper thawing procedures and avoid prolonged exposure of the serum to high temperatures. To avoid repeated freeze-thaw cycles, the 50 mL format is recommended for ready-to-use convenience; ten 50 mL bottles are equivalent to one 500 mL bottle. If precipitates form during thawing, aliquot into sterile centrifuge tubes, centrifuge at 400–600 g (e.g., 500 g) for 5 min, add the supernatant to the basal medium, and filter together under aseptic conditions. |
| Batch-to-Batch Consistency | Contains multiple growth factors with stable batch-to-batch performance, ensuring consistent cell culture status. |
| Heat Inactivation | The serum has been heat-inactivated prior to release; additional heat inactivation by the end user is generally not required. |
For research use only, not for clinical use.
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