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| Product Name | Ferrous Iron Colorimetric Assay Kit |
| Catalog No. | ETK-YJL-0002 |
| Size | 96 T (80 samples), 48 T (32 samples) |
| Sample Type | Serum (plasma); animal and plant tissues |
| Detection Instrument | Microplate reader (590-600 nm, optimal detection wavelength: 593 nm) |
| Research Field | Inorganic salt ions; Plant physiology series; Ferroptosis |
| Sensitivity | 0.4 μmol/L |
| Detection Range | 0.4-50 μmol/L |
| Accuracy | Inter-assay variation: 1.5% | Intra-assay variation: 1.3% |
| Sample Size | 55 μL(serum/plasma), 300 μL(tissue homogenate) |
| Storage | The product can be stored at 2-8°C, protected from light for 6 months. |
| Shelf Life | 6 months |
| Detection Time | 40 min |
Ferrous iron (Fe²⁺) is an essential trace element involved in numerous critical biological processes across both prokaryotic and eukaryotic organisms. Its unique redox properties make it a key component of various metalloenzymes, including cytochromes, hemoglobin, myoglobin, and ribonucleotide reductase, which are vital for oxygen transport, energy metabolism, DNA synthesis, and electron transfer chains. Beyond these fundamental roles, ferrous iron also plays a pivotal role in regulating cellular signaling pathways and maintaining redox homeostasis within cells.
However, the delicate balance of intracellular ferrous iron levels is crucial for biological function. Dysregulation of ferrous iron metabolism can lead to severe physiological consequences:
Excessive accumulation of free ferrous iron triggers the Fenton reaction, generating reactive oxygen species (ROS) such as hydroxyl radicals. These highly toxic molecules cause oxidative damage to lipids, proteins, and nucleic acids, contributing to cellular dysfunction and tissue injury.
Iron overload is associated with the pathogenesis of various diseases, including hemochromatosis, liver cirrhosis, and neurodegenerative disorders like Alzheimer’s and Parkinson’s disease.
Conversely, insufficient ferrous iron availability results in iron deficiency anemia, impairing oxygen delivery and leading to fatigue, weakness, and compromised immune function.
In the field of scientific research, accurate and reliable quantification of ferrous iron is indispensable for investigating iron metabolism mechanisms, exploring the role of iron in disease progression, and evaluating the efficacy of iron-modulating therapies. It is also a critical parameter in studies related to ferroptosis—a newly identified form of regulated cell death driven by iron-dependent lipid peroxidation. The demand for sensitive, specific, and user-friendly assay tools to measure ferrous iron in diverse biological samples has therefore grown exponentially in recent years, making high-quality ferrous iron detection kits a cornerstone of modern biomedical and life science research.
Broad sample compatibility, supporting the detection of ferrous iron in serum, plasma, and various animal and plant tissue homogenates, meeting the needs of multi-disciplinary research.
Optimal detection wavelength set at 593 nm (compatible with 590-600 nm microplate readers), ensuring strong signal specificity and minimizing interference from non-target substances.
High sensitivity with a lower detection limit of 0.4 μmol/L, enabling the accurate quantification of low-abundance ferrous iron in trace samples.
Wide detection range of 0.4-50 μmol/L, covering the physiological and pathological concentration ranges of ferrous iron in most biological samples, eliminating the need for repeated sample dilution or concentration.
Excellent precision, with inter-assay variation of only 1.5% and intra-assay variation of 1.3%, guaranteeing consistent and reliable results across multiple experiments and batches.
Minimal sample requirement (55 μL for serum/plasma, 300 μL for tissue homogenate), conserving valuable biological samples especially for rare or hard-to-obtain specimens.
Rapid detection process completed within 40 minutes, significantly reducing experimental time and improving research efficiency.
Stable storage conditions (2-8°C, protected from light) with a 6-month shelf life, ensuring product performance integrity during long-term storage and transportation.
User-friendly operation protocol, suitable for both experienced researchers and laboratory beginners, without the need for complex equipment or specialized technical training.
Superior accuracy and reproducibility: The low inter-assay and intra-assay variation rates ensure that experimental data is trustworthy and statistically valid, reducing the risk of erroneous conclusions caused by measurement errors.
Balanced sensitivity and dynamic range: Effectively detects both low and high concentrations of ferrous iron without sacrificing accuracy, adapting to the diverse concentration gradients of samples in different research scenarios.
Time and cost-efficient: The 40-minute rapid assay workflow saves laboratory time, while the optimized reagent formulation reduces the need for additional experimental materials, lowering overall research costs.
Robust sample tolerance: The assay system is designed to resist interference from common substances in biological samples (e.g., proteins, lipids, metal ions), ensuring reliable results even with complex sample matrices.
Versatile application scope: Ideal for research in inorganic salt ion analysis, plant physiology, ferroptosis, iron metabolism, and disease-related studies, supporting multi-faceted scientific investigations.
Consistent product quality: Each batch of the kit undergoes strict quality control testing to ensure uniform performance, providing researchers with stable experimental conditions.
Space-saving storage: No requirement for ultra-low temperature freezers, simplifying laboratory storage management and reducing equipment investment.
Comprehensive technical support: Backed by professional technical teams to assist with experimental troubleshooting, ensuring a smooth research process for users.
For research use only, not for clinical use.
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