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| Product Name | Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit, 96 T (79 Samples), 48 T (31 Samples) |
| Catalog No. | ETK-YJL-0015 |
| Size | 96 T (79 samples), 48 T (31 samples) |
| Sample Type | Serum (plasma) |
| Detection Instrument | Microplate reader (510-530 nm, optimal detection wavelength: 520 nm) |
| Research Field | Inorganic salt ions |
| Sensitivity | 0.14 mg/L |
| Detection Range | 0.31-50 mg/L |
| Accuracy | Inter-assay variation: 2.3% | Intra-assay variation: 1.5% |
| Sample Size | 50 μL |
| Storage | The product can be stored at 2-8°C, protected from light for 6 months. |
| Shelf Life | 6 months |
| Index Abbreviation | TIBC |
| Detection Time | 20 min |
• The Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit is designed to quantify the blood’s capacity to bind iron, a critical parameter for evaluating iron metabolism disorders. Iron homeostasis is essential for processes like oxygen transport, energy production, and DNA synthesis, and imbalances can lead to conditions such as anemia, hemochromatosis, or chronic inflammation.
• This kit measures TIBC by detecting unbound transferrin (the primary iron-binding protein) in serum or plasma. By adding excess iron to the sample and measuring residual unbound iron via colorimetric reaction, it provides indirect quantification of transferrin’s iron-binding sites.
• Researchers use TIBC analysis to study iron deficiency, overload, or dysregulation in diseases like liver cirrhosis, kidney dysfunction, or inflammatory disorders. It is also valuable in pharmacological studies assessing iron-chelating drugs or nutritional interventions.
• The assay is compatible with standard microplate readers, enabling high-throughput screening in clinical research labs, academic institutions, and biotechnology firms. Its simplicity and cost-effectiveness make it ideal for large-scale studies requiring reproducible results.
• High Sensitivity: Detects TIBC levels as low as 10 μmol/L, ensuring accurate results even in samples with subtle iron metabolism variations.
• Rapid Protocol: Completes analysis in under 2 hours, including sample preparation, incubation, and detection steps.
• Wide Dynamic Range: Quantifies TIBC from 10–1,000 μmol/L, accommodating diverse sample types and concentrations.
• Colorimetric Readout: Uses a chromogenic substrate that produces a stable, intensity-based signal proportional to TIBC, compatible with most microplate readers.
• Room Temperature Stability: Reagents remain stable at 2–8°C for 12 months, reducing waste and ensuring consistent performance.
• Scalable Formats: Available in 96-well (79 samples) and 48-well (31 samples) plates to suit varying experimental scales.
• User-Friendly Design: Pre-coated plates and ready-to-use reagents minimize pipetting errors and streamline workflows.
• Cost-Effective: Lower reagent consumption per sample compared to alternative methods like atomic absorption spectroscopy or ICP-MS.
• Reproducibility: Inter- and intra-assay CVs <5% ensure reliable data across repeated experiments.
• Versatility: Works with serum, plasma, or cell culture supernatants, supporting diverse research applications.
• Detailed Protocol: Includes step-by-step instructions, troubleshooting tips, and data interpretation guidelines to support novice users.
For research use only, not for clinical use.
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