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| Product Name | Phosphorus (Pi) Colorimetric Assay Kit (Phospho Molybdate Method), 100 Assays (96 Samples), 50 Assays (48 Samples) |
| Catalog No. | ETK-YJL-0017 |
| Size | 100 Assays (96 samples), 50 Assays (48 samples) |
| Sample Type | Serum (plasma); tissues |
| Detection Instrument | UV-Visible Spectrophotometer (660 nm) |
| Research Field | Inorganic salt ions; Plant physiology series |
| Sensitivity | 0.005 mmol/L |
| Detection Range | 0.005-2.0 mmol/L |
| Accuracy | Inter-assay variation: 1.3% | Intra-assay variation: 0.01 |
| Sample Size | 0.1 mL |
| Storage | The product can be stored at 2-8°C, protected from light for 6 months. |
| Shelf Life | 6 months |
| Index Abbreviation | Pi |
| Detection Time | 1 h |
Phosphorus (Pi) is an essential inorganic salt ion involved in numerous critical biological processes, including energy metabolism (e.g., ATP synthesis), nucleic acid synthesis, signal transduction, and cell membrane structure formation. Accurate quantification of Pi levels is fundamental in various research fields, such as plant physiology, microbial metabolism, cell biology, and biochemistry.
In plant physiology research, Pi plays a pivotal role in photosynthesis, nutrient uptake, and stress response. Deficiencies or excesses of Pi can significantly impact plant growth, development, and yield, making Pi detection a key tool for studying plant nutrient management and environmental adaptation.
Traditional Pi detection methods often suffer from limitations such as low sensitivity, complex operation, long detection cycles, or interference from other substances. The phospho-molybdate colorimetric method has emerged as a reliable and widely adopted technique for Pi quantification due to its simplicity, specificity, and compatibility with standard laboratory equipment.
This Phosphorus (Pi) Colorimetric Assay Kit is designed to meet the growing demand for accurate, efficient, and reproducible Pi analysis in research settings, providing researchers with a user-friendly solution to obtain precise Pi concentration data for their experiments.
Utilizes the classic phospho-molybdate colorimetric principle, ensuring high specificity for Pi without significant interference from common biological molecules.
Offers a wide detection range of 0.005–2.0 mmol/L, covering the Pi concentration levels typically encountered in serum (plasma), tissues, and other research samples.
Boasts high sensitivity with a lower detection limit of 0.005 mmol/L, enabling the quantification of low-abundance Pi in dilute samples.
Requires only 0.1 mL of sample volume, minimizing sample consumption and preserving valuable research materials.
Completed detection within 1 hour, providing rapid results to accelerate experimental progress.
Compatible with UV-Visible Spectrophotometers at 660 nm, a standard instrument in most research laboratories, eliminating the need for specialized or expensive equipment.
Stable storage at 2–8°C in the dark for 6 months, ensuring long-term usability and convenient inventory management.
Exceptional accuracy with low inter-assay variation (1.3%) and intra-assay variation, guaranteeing reliable and reproducible results across experiments and between different operators.
User-friendly protocol with straightforward steps, reducing the learning curve and suitable for both experienced researchers and laboratory beginners.
Consistent performance across multiple sample types (serum/plasma and tissues), offering versatility for diverse research applications.
Pre-optimized reagents and standardized procedures minimize experimental errors, ensuring consistent data quality and reducing the need for repeated tests.
Cost-effective assay design, providing sufficient capacity for 50 or 100 assays (corresponding to 48 or 96 samples) to meet the needs of small-scale experiments and large-scale screening projects alike.
For research use only, not for clinical use.
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