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| Product Name | Phosphorus (Pi) Colorimetric Assay Kit (Phospho Molybdate Method), 96 T (80 Samples), 48 T (32 Samples) |
| Catalog No. | ETK-YJL-0010 |
| Size | 96 T (80 samples), 48 T (32 samples) |
| Sample Type | Serum (plasma); tissues |
| Detection Instrument | Microplate reader (620-690 nm, optimal detection wavelength: 660 nm) |
| Research Field | Inorganic salt ions; Plant physiology series |
| Sensitivity | 0.004 mmol/L |
| Detection Range | 0.004-2.0 mmol/L |
| Accuracy | Inter-assay variation: 0.03 | Intra-assay variation: 2.1% |
| Sample Size | 0.1 mL |
| Storage | The product can be stored at 2-8°C, protected from light for 6 months. |
| Shelf Life | 6 months |
| Index Abbreviation | Pi |
| Detection Time | 40 min |
Phosphorus (Pi) is an essential inorganic salt ion involved in numerous critical biological processes across both plant and animal systems. In plant physiology, it plays a pivotal role in photosynthesis, energy transfer (via ATP and ADP), nucleic acid synthesis, and nutrient transport, directly influencing plant growth, development, and stress resistance. For research involving serum, plasma, and tissue samples, accurate quantification of Pi is vital to understanding metabolic pathways, cellular signaling, and pathological mechanisms related to conditions such as mineral metabolism disorders.
Traditional methods for Pi detection often suffer from limitations like low sensitivity, long assay times, or interference from other biological molecules. The Phospho Molybdate Method addresses these challenges by leveraging a highly specific reaction between inorganic phosphorus and molybdate under optimized conditions, producing a colored complex that can be precisely measured spectrophotometrically. This method has become a trusted choice for researchers in inorganic salt ion analysis and plant physiology due to its reliability and ease of use.
This Phosphorus (Pi) Colorimetric Assay Kit is designed to meet the growing demand for rapid, accurate, and reproducible Pi quantification in research settings. It is tailored for serum (plasma) and tissue samples, providing a standardized workflow that minimizes experimental variability and ensures consistent results across studies.
Targeted sample compatibility: Optimized for serum (plasma) and tissue samples, covering key research matrices in plant physiology and inorganic salt ion studies.
Broad detection range: Measures Pi concentrations from 0.004 mmol/L to 2.0 mmol/L, accommodating both low and high Pi levels in diverse research samples.
High sensitivity: Detects as low as 0.004 mmol/L of Pi, enabling the quantification of trace amounts of inorganic phosphorus in samples with low Pi content.
Rapid assay process: Completes the entire detection procedure in just 40 minutes, saving valuable research time compared to lengthier traditional methods.
Simple operation: Follows a straightforward workflow that requires minimal technical expertise, suitable for both experienced researchers and laboratory technicians.
Stable storage conditions: Can be stored at 2-8°C protected from light for 6 months, ensuring long-term usability and reducing the need for frequent reordering.
Exceptional accuracy: Features low inter-assay variation (0.03) and intra-assay variation (2.1%), guaranteeing reliable and consistent results across multiple experiments and sample batches.
Optimal detection wavelength: Calibrated for use with a microplate reader at 620-690 nm (optimal 660 nm), a commonly available instrument in research laboratories, eliminating the need for specialized equipment.
Minimal sample requirement: Only 0.1 mL of sample is needed per assay, conserving precious or limited research samples (e.g., rare tissue specimens or small-volume serum samples).
Specific reaction mechanism: The Phospho Molybdate Method exhibits high specificity for inorganic phosphorus, reducing interference from other biological components in serum, plasma, and tissue homogenates.
Cost-effective packaging options: Available in 96 T (80 samples) and 48 T (32 samples) formats, allowing researchers to choose the size that best fits their experimental scale and budget.
Quality-controlled components: All kit reagents are rigorously tested to ensure batch-to-batch consistency, minimizing experimental errors and enhancing data reproducibility.
For research use only, not for clinical use.
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