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Chlorine (Cl) Colorimetric Assay Kit

Cat.No: ETK-YJL-0011 Datasheet

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Product Name Chlorine (Cl) Colorimetric Assay Kit
Catalog No. ETK-YJL-0011
Size 96 T (80 samples)
Sample Type Serum (plasma); animal tissues
Detection Instrument Microplate reader (440-480 nm, optimal detection wavelength: 460 nm)
Research Field Inorganic salt ions; Plant physiology series
Sensitivity 1 mmol/L
Detection Range 1.0-60 mmol/L
Accuracy Inter-assay variation: 6.4% | Intra-assay variation: 3.6%
Sample Size 10 μL
Storage The product can be stored at 2-8°C, protected from light for 6 months.
Shelf Life 6 months
Index Abbreviation Cl
Detection Time 30 min

Chlorine (Cl⁻) is an essential inorganic anion that plays critical roles in multiple biological, environmental, and industrial systems, making accurate and efficient detection of chlorine levels a fundamental need across diverse fields.

In biological research, chlorine ions are vital for maintaining cellular homeostasis—they regulate osmotic pressure in animal tissues, support nerve impulse transmission, and participate in acid-base balance in serum and plasma. For researchers studying plant physiology, chlorine also acts as a micronutrient, influencing processes like photosynthesis and stomatal function; abnormal chlorine levels in plant tissues can indicate stress, nutrient deficiencies, or environmental adaptability issues. Thus, reliable measurement of chlorine in serum, plasma, and animal tissues is indispensable for advancing studies in physiology, pathology, and pharmacology, such as investigating electrolyte imbalances in disease models or evaluating the impact of experimental treatments on ion metabolism.

Beyond biological research, chlorine detection is equally critical in environmental monitoring and industrial quality control. In water treatment (drinking water, wastewater, and cooling towers), chlorine is widely used as a disinfectant to eliminate pathogens, but excessive residual chlorine can form harmful disinfection by-products (DBPs) that pose risks to human health and aquatic ecosystems. In the food and beverage industry, chlorine-based sanitizers are used to ensure rinse water safety, requiring precise monitoring to balance disinfection efficacy and product safety. Additionally, in chemical analysis and manufacturing, chlorine levels serve as a key quality indicator for processes involving chloride-containing compounds, preventing process errors and ensuring end-product compliance with standards.

Traditional methods for chlorine detection, such as titration or ion chromatography, often suffer from limitations: titration is labor-intensive, requires skilled operation, and has low throughput; ion chromatography, while accurate, demands expensive equipment, long analysis times, and specialized maintenance—making it unsuitable for high-volume sample testing or on-site applications. To address these gaps, the Chlorine (Cl) Colorimetric Assay Kit was developed. Leveraging a highly specific colorimetric reaction principle, this kit combines simplicity, speed, and precision, enabling researchers and professionals to quantify chlorine concentrations efficiently without relying on complex instruments. It is tailored to meet the growing demand for accessible, high-performance chlorine detection tools, bridging the gap between laboratory research needs and real-world application requirements.

Broad Sample Compatibility: Designed to analyze chlorine levels in common biological samples, including serum, plasma, and various animal tissues (e.g., liver, kidney, and spleen). This versatility eliminates the need for multiple specialized kits, simplifying workflow for researchers working across different sample types.
Optimized Detection Wavelength and Instrument Compatibility: Operates at a detection wavelength range of 440-480 nm, with an optimal wavelength of 460 nm—compatible with standard microplate readers widely available in most laboratories. This ensures easy integration into existing experimental setups without the need for additional specialized equipment.
Fast Turnaround Time: Completes the entire assay process in just 30 minutes, significantly reducing waiting time compared to traditional methods (e.g., ion chromatography, which may take hours). This high efficiency is ideal for labs handling large sample batches or time-sensitive experiments.
Low Sample Requirement: Only requires 10 μL of sample per test. This minimizes sample consumption, a critical advantage when working with limited or precious samples (e.g., small-volume animal serum or rare tissue homogenates).
Stable Storage and Long Shelf Life: Can be stored at 2-8°C under light-protected conditions for 6 months. This stable storage profile reduces the need for frequent kit replacements, lowers waste, and ensures consistent performance over an extended period.

High Accuracy with Low Variation: Delivers reliable results with inter-assay variation of only 6.4% and intra-assay variation of 3.6%. Low variation ensures that data is consistent across different experiments and operators, enhancing the reproducibility of research findings—a key requirement for scientific publications and industrial quality control.
Well-Defined Detection Range and Sensitivity: Offers a detection range of 1.0-60 mmol/L and a sensitivity of 1 mmol/L, which aligns with the typical chlorine concentration ranges in biological samples (e.g., normal serum chlorine levels in mammals are ~96-106 mmol/L, and diluted tissue homogenates fall within the kit’s range). This ensures that both low and moderate chlorine levels can be accurately quantified without the need for excessive sample dilution or concentration.
User-Friendly Protocol: Requires no complex pre-treatment steps (e.g., sample digestion or purification) and includes all necessary reagents and a detailed instruction manual. The straightforward workflow is accessible to both seasoned researchers and novice users, reducing training time and minimizing human error during operation.
Cost-Effective High-Throughput Design: Provides 96 test wells (supporting 80 samples) per kit, offering a low cost per test. This makes it economical for labs conducting large-scale screenings (e.g., testing chlorine levels in multiple tissue samples from a cohort of experimental animals) or routine quality checks.
Robust Performance in Complex Matrices: The colorimetric reaction principle is resistant to interference from common contaminants in biological samples (e.g., proteins, other ions). This robustness ensures accurate results even when analyzing complex tissue homogenates or serum samples with varying compositions, eliminating the need for additional cleanup steps.

For research use only, not for clinical use.

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