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| Product Name | Magnesium (Mg) Colorimetric Assay Kit, 100 Assays (96 Samples), 50 Assays (48 Samples) |
| Catalog No. | ETK-YJL-0013 |
| Size | 100 Assays (96 samples), 50 Assays (48 samples) |
| Sample Type | Serum (plasma) |
| Detection Instrument | UV-Visible Spectrophotometer (540 nm) |
| Research Field | Inorganic salt ions; Plant physiology series |
| Sensitivity | 0.12 mmol/L |
| Detection Range | 0.12-2.50 mmol/L |
| Accuracy | Inter-assay variation: 9.9% | Intra-assay variation: 4.8% |
| Sample Size | 10 μL |
| Storage | The product can be stored at 2-8°C, protected from light for 6 months. |
| Shelf Life | 6 months |
| Index Abbreviation | Mg |
| Detection Time | 50 min |
Magnesium (Mg) is an indispensable inorganic cation in living organisms and holds a pivotal position in numerous biological processes that underpin life activities. As the second most abundant intracellular cation in the human body and a key component of plant chlorophyll, its role spans across both animal and plant biological systems, making it a core focus in the fields of inorganic salt ions research and plant physiology.
In animal biology, magnesium is involved in over 300 enzymatic reactions, acting as a critical cofactor to regulate metabolic pathways. It plays a vital role in energy metabolism by facilitating the conversion of adenosine triphosphate (ATP) — the cell’s primary energy currency — into usable energy, which is essential for muscle contraction, nerve signal transmission, and the synthesis of nucleic acids (DNA and RNA). For instance, in nerve cells, magnesium ions help maintain the resting membrane potential, preventing abnormal nerve excitation and ensuring stable neural function. In muscle tissue, it coordinates with calcium ions to control muscle relaxation and contraction cycles, avoiding issues like muscle spasms or weakness caused by magnesium deficiency. Additionally, magnesium contributes to maintaining normal heart rhythm, supporting immune system function, and regulating bone mineralization, as it works in tandem with calcium and phosphorus to build and maintain bone density.
In the context of plant physiology, magnesium is the central atom of chlorophyll, the pigment responsible for capturing light energy during photosynthesis. Without sufficient magnesium, plants cannot efficiently synthesize chlorophyll, leading to chlorosis (yellowing of leaves), reduced photosynthetic efficiency, and ultimately stunted growth or decreased crop yields. Beyond photosynthesis, magnesium also participates in plant enzyme activation, carbohydrate metabolism, and the transport of nutrients within the plant. For example, it activates enzymes involved in the synthesis of sucrose, which is crucial for the translocation of energy from leaves to other plant organs like roots, fruits, and seeds.
Given the multifaceted role of magnesium in biological systems, accurate and efficient detection of magnesium concentrations in biological samples is of great significance for scientific research. In medical and physiological research, measuring serum or plasma magnesium levels helps study the relationship between magnesium imbalance and diseases such as cardiovascular disorders, neurological disorders, and metabolic syndromes. In plant science research, quantifying magnesium in plant tissues or growth media aids in understanding plant nutrient uptake mechanisms, optimizing fertilization strategies, and investigating the impact of environmental stress (e.g., drought, soil salinity) on plant magnesium metabolism.
Traditional methods for magnesium detection, such as atomic absorption spectroscopy, often require expensive equipment, complex sample pretreatment, and specialized operational skills, which limit their accessibility and efficiency in routine research. The Magnesium (Mg) Colorimetric Assay Kit addresses these challenges by leveraging a simple, reliable colorimetric principle. It enables researchers to quickly and accurately determine magnesium concentrations in serum or plasma samples using a common UV-Visible Spectrophotometer, eliminating the need for advanced instrumentation and cumbersome sample preparation. This kit is specifically designed to meet the needs of researchers in the fields of inorganic salt ions and plant physiology, providing a cost-effective and user-friendly tool to support their studies on magnesium-related biological processes.
Broad Sample Compatibility for Target Research Fields: The kit is optimized for serum and plasma samples, which are the most commonly used biological matrices in inorganic salt ions research (e.g., studying animal magnesium metabolism) and plant physiology research (e.g., analyzing magnesium transport in plant-derived biological fluids). This focus ensures high reliability and accuracy for samples relevant to the kit’s core application areas.
Precise Detection Performance: It offers a low sensitivity of 0.12 mmol/L, allowing for the detection of low magnesium concentrations in samples — a critical capability for research scenarios such as studying magnesium deficiency in early-stage biological models. The detection range of 0.12-2.50 mmol/L covers the typical physiological concentration range of magnesium in serum and plasma, ensuring that most research samples can be measured without excessive dilution.
Controlled Assay Variation for Reproducible Results: With an inter-assay variation of 9.9% and an intra-assay variation of 4.8%, the kit minimizes experimental errors caused by batch-to-batch differences and operational fluctuations. This high level of precision is essential for scientific research, as it ensures that results are consistent and reliable across multiple experiments, supporting the validity of research conclusions.
Time-Efficient Experimental Workflow: The total detection time is only 50 minutes, which significantly shortens the experimental cycle compared to traditional detection methods. This efficiency allows researchers to process more samples within a shorter period, accelerating the progress of research projects related to magnesium analysis.
User-Friendly Sample Requirements: The kit requires only 10 μL of sample per assay, reducing the demand for large sample volumes. This is particularly beneficial for research where sample availability is limited, such as studies using small animal models (e.g., mice, rats) or rare plant-derived biological fluids.
Convenient Storage and Long Shelf Life: The kit can be stored at 2-8°C (a standard refrigeration temperature) and protected from light for 6 months. This eliminates the need for specialized storage conditions (e.g., -20°C freezers) and ensures a long shelf life, making it easy for laboratories to stock and use the kit without frequent replacements.
Simple Instrumentation Dependence: It uses a UV-Visible Spectrophotometer at 540 nm — a common piece of equipment in most research laboratories. Researchers do not need to invest in expensive, specialized instruments, reducing the barrier to entry for using the kit and making it accessible to a wide range of research teams.
Research-Centric Design Aligned with Core Application Needs: Unlike general-purpose magnesium detection kits, this product is tailored specifically for the fields of inorganic salt ions and plant physiology. Its performance parameters (e.g., sample type compatibility, detection range) are optimized to address the unique research questions in these areas, ensuring that it provides meaningful and accurate data for studies such as magnesium ion homeostasis in animals or magnesium’s role in plant photosynthesis.
Superior Sensitivity for Low-Concentration Research: With a sensitivity of 0.12 mmol/L, it outperforms some competing kits (e.g., those with a sensitivity of 0.18 mmol/L) in detecting low magnesium levels. This advantage is crucial for research focusing on subtle changes in magnesium concentration, such as tracking early magnesium depletion in biological systems or measuring trace magnesium in plant sap.
Cost-Effective Assay Capacity: The kit is available in two sizes — 100 assays (for 96 samples) and 50 assays (for 48 samples) — allowing laboratories to choose the appropriate size based on their sample throughput. This flexibility avoids unnecessary waste of reagents and reduces the per-assay cost, making it a cost-effective solution for both small-scale experiments and large-scale research projects.
Minimized Experimental Burden with Low Sample Volume: Requiring only 10 μL of sample per assay, the kit reduces the need to collect large quantities of samples, which is especially valuable for research involving invasive sample collection (e.g., animal blood sampling) or samples that are difficult to obtain (e.g., rare plant varieties). This not only simplifies sample collection procedures but also minimizes stress on experimental subjects, aligning with ethical research practices.
Stable Performance for Consistent Research Outcomes: The low intra-assay variation (4.8%) ensures that repeated measurements of the same sample yield consistent results, reducing the need for repeated experiments and saving time and resources. The 6-month shelf life under standard refrigeration conditions also guarantees that the kit maintains its performance over time, so researchers can rely on its accuracy throughout the duration of their projects.
Easy Integration into Existing Laboratory Workflows: Since the kit uses a common UV-Visible Spectrophotometer and does not require complex sample pretreatment, it can be easily integrated into the existing experimental workflows of most research laboratories. Researchers do not need to undergo extensive training to use the kit, reducing the learning curve and enabling quick adoption.
For research use only, not for clinical use.
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