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| Product Name | Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit, 50 Assays (48 Samples) |
| Catalog No. | ETK-YJL-0016 |
| Size | 50 Assays (48 samples) |
| Sample Type | Serum (plasma) |
| Detection Instrument | UV-Visible Spectrophotometer (520 nm) |
| Research Field | Inorganic salt ions |
| Sensitivity | 0.03 mg/L |
| Detection Range | 0.03-50 mg/L |
| Accuracy | Inter-assay variation: 4.7% | Intra-assay variation: 3.4% |
| Sample Size | 1 mL |
| Storage | The product can be stored at 2-8°C, protected from light for 6 months. |
| Shelf Life | 6 months |
| Index Abbreviation | TIBC |
| Detection Time | 50 min |
• The Total Iron Binding Capacity (TIBC) Colorimetric Assay Kit is designed for quantifying the blood’s capacity to bind iron, a critical parameter in evaluating iron metabolism disorders such as anemia, hemochromatosis, and chronic kidney disease.
• Iron homeostasis is tightly regulated by proteins like transferrin, which binds iron for transport to tissues. TIBC reflects the amount of transferrin available to bind iron, providing insights into iron deficiency or overload.
• This assay kit is part of a broader suite of biochemical tools used in hematology, biochemistry, and clinical research to study iron-related pathways, nutritional deficiencies, and systemic iron regulation.
• Researchers utilize TIBC measurements alongside serum iron and ferritin levels to diagnose conditions like iron-deficiency anemia (low TIBC) or iron overload (high TIBC), enabling targeted therapeutic interventions.
• The colorimetric method employed in this kit offers a rapid, cost-effective alternative to automated analyzers, making it suitable for laboratories with limited resources or high-throughput screening needs.
• High Sensitivity: Detects TIBC levels as low as 5 μmol/L, ensuring accurate quantification even in samples with marginal iron-binding capacity.
• Wide Dynamic Range: Measures TIBC concentrations from 5–1,000 μmol/L, accommodating diverse sample types and physiological conditions.
• Colorimetric Readout: Utilizes a chromogenic substrate that produces a stable, intensity-based color change proportional to TIBC, compatible with standard microplate readers.
• Room Temperature Stability: Reagents remain stable at 2–8°C for 12 months, reducing waste and ensuring consistent performance over time.
• Streamlined Protocol: Requires only 3 simple steps—sample incubation, reagent addition, and absorbance measurement—minimizing hands-on time and technical variability.
• Time-Efficient: Results are generated within 60 minutes, enabling same-day data analysis and faster decision-making in research workflows.
• Cost-Effective: Priced competitively, this kit reduces per-sample costs compared to automated analyzers, making it ideal for budget-conscious laboratories.
• User-Friendly: No specialized equipment or training is required beyond a microplate reader, lowering the barrier to entry for novice researchers.
• High Reproducibility: Internal quality controls ensure batch-to-batch consistency, minimizing inter-assay variability and enhancing data reliability.
• Scalable: Supports 50 assays (48 samples + 2 standards/controls), allowing flexibility for small-scale pilot studies or large-scale screening projects.
For research use only, not for clinical use.
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