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| Product Name | Fast Restriction Enzyme MunI (MfeI) (Recognition Site: 5'-C↓AATTG-3') |
| Catalog No. | IVDRM-0056 |
| Description | This fast restriction enzyme is a recombinant endonuclease engineered for speed and precision, delivering complete DNA cleavage in just 5–15 minutes. It is well suited for the rapid digestion of plasmid DNA, PCR products, and genomic DNA. Every enzyme in the series works in the same universal digestion buffer, greatly simplifying reaction setup, and provides ample activity headroom to tackle excess substrate or difficult templates with confidence. Fast Restriction Enzyme MunI (also known as MfeI) recognizes the sequence 5'-C↓AATTG-3' (3'-GTTAA↑C-5') and is supplied at 20 U/μL. Digestion is performed at 37 °C in a single universal buffer. MunI cannot be heat-inactivated; use phenol-chloroform extraction or column purification to remove the enzyme. Its activity is unaffected by Dam, Dcm, CpG, EcoKI, or EcoBI methylation. |
| Intended Use | For the rapid digestion of plasmid DNA, PCR products, and genomic DNA in molecular cloning and related molecular biology workflows. |
| Principle / Technology | A recombinant type II restriction endonuclease produced by genetic engineering. It recognizes a specific double-stranded DNA sequence and cleaves both strands at defined positions, completing precise digestion within 5–15 minutes. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA. |
| Performance Range / Specifications | Complete, precise DNA digestion in 5–15 minutes. Generous activity overcapacity easily handles excess substrate or difficult templates. |
| Specificity | Specifically recognizes 5'-C↓AATTG-3' and cleaves between C and A (3'-GTTAA↑C-5' on the complementary strand). |
| Reaction Conditions / Protocol | Recommended reaction conditions: 1× universal fast digestion buffer; incubate at the optimal temperature of 37 °C for 5–15 minutes. |
| Storage Conditions | Store at -25 to -15 °C. |
| Shelf Life | 2 years. |
| Package Specifications | 25 T |
| Concentration | 20 U/μL |
| Source / Origin | Recombinant enzyme produced by genetic engineering technology. |
| Expiration Date / Stability | Stable for 2 years when stored at -25 to -15 °C. |
| Compatibility | MfeI |
| Recommended Buffer System | All fast restriction enzymes in this series share a single universal fast digestion buffer (1×), which streamlines and simplifies the digestion reaction setup. |
| Application Notes / Precautions | MunI cannot be heat-inactivated; remove the enzyme by phenol-chloroform extraction or column purification before downstream applications. The shared universal buffer simplifies the digestion system; the enzyme offers good activity overcapacity for digesting excess substrate or difficult templates. |
| Recognition Site | 5'-C↓AATTG-3' 3'-GTTAA↑C-5' |
| Heat Inactivation | Cannot be heat-inactivated; use phenol-chloroform extraction or column purification. |
| Methylation Sensitivity | Dam, Dcm, CpG, EcoKI, EcoBI: no effect. |
For research use only, not for clinical use.
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