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| Product Name | Fast Restriction Enzyme MseI (Recognition Site: T↓TAA) |
| Catalog No. | IVDRM-0057 |
| Description | Alta DiagnoTech fast restriction enzymes are recombinant enzymes produced by genetic engineering, delivering precise DNA cleavage within 5–15 minutes. They are ideal for rapid digestion of plasmid DNA, PCR products, and genomic DNA. All enzymes in the series share a single universal digestion buffer, greatly simplifying reaction setup, and provide ample activity headroom to handle excess substrate or difficult templates with ease. |
| Intended Use | For rapid restriction digestion of plasmid DNA, PCR products, and genomic DNA in molecular cloning workflows. For research use only. |
| Principle / Technology | Recombinant type II restriction endonuclease produced by genetic engineering. It recognizes a specific palindromic DNA sequence and cleaves both strands at defined positions, enabling accurate DNA digestion within 5–15 minutes. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Complete, precise DNA digestion within 5–15 minutes. High activity redundancy allows efficient processing of excess substrate or difficult templates. |
| Specificity | Specifically recognizes the sequence 5'-T↓TAA-3' and cleaves at defined positions, generating precise, reproducible fragments without star activity. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; incubate at 37°C. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 2 years when stored at -25°C to -15°C. |
| Package Specifications | 50 T |
| Concentration | 10 U/μL |
| Source / Origin | Recombinant enzyme produced by genetic engineering. |
| Expiration Date / Stability | Stable for 2 years when stored at -25°C to -15°C. |
| Compatibility | Isoschizomers: Tru1I, Tru9I, SaqAI |
| Recommended Buffer System | All fast restriction enzymes in this series share a single universal fast digestion buffer, simplifying multi-enzyme reaction setup. |
| Application Notes / Precautions | Use 1× universal fast digestion buffer for optimal performance; the same buffer is shared by all fast restriction enzymes in this series. The enzyme offers high activity redundancy, so excess substrate or difficult templates can be digested without adjustment. Incubate at 37°C; typical digestion is complete within 5–15 minutes. |
| Recognition Site | 5'-T↓TAA-3' 3'-AAT↑T-5' |
| Heat Inactivation | Incubate at 80°C for 20 min. |
| Methylation Sensitivity | EcoKI: cleavage blocked Dam, Dcm, CpG, EcoBI: no effect |
For research use only, not for clinical use.
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