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| Product Name | Fast Restriction Enzyme MluI (Recognition Site: 5'-A↓CGCGT-3') |
| Catalog No. | IVDRM-0053 |
| Description | This fast restriction enzyme is a recombinant endonuclease engineered for speed and precision, delivering complete DNA cleavage in just 5–15 minutes. It is well suited for the rapid digestion of plasmid DNA, PCR products, and genomic DNA. Every enzyme in the series works in the same universal digestion buffer, greatly simplifying reaction setup, and provides ample activity headroom to tackle excess substrate or difficult templates with confidence. Fast Restriction Enzyme MluI recognizes the sequence 5'-A↓CGCGT-3' (3'-TGCGC↑A-5') and is supplied at 20 U/μL. Digestion is performed at 37 °C in a single universal buffer, and the enzyme can be heat-inactivated at 80 °C for 20 minutes. Note that CpG methylation fully overlapping the recognition sequence blocks cleavage. |
| Intended Use | For the rapid digestion of plasmid DNA, PCR products, and genomic DNA in molecular cloning and related molecular biology workflows. |
| Principle / Technology | A recombinant type II restriction endonuclease produced by genetic engineering. It recognizes a specific double-stranded DNA sequence and cleaves both strands at defined positions, completing precise digestion within 5–15 minutes. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA. |
| Performance Range / Specifications | Complete, precise DNA digestion in 5–15 minutes. Generous activity overcapacity easily handles excess substrate or difficult templates. |
| Specificity | Specifically recognizes 5'-A↓CGCGT-3' and cleaves between A and C (3'-TGCGC↑A-5' on the complementary strand). |
| Reaction Conditions / Protocol | Recommended reaction conditions: 1× universal fast digestion buffer; incubate at the optimal temperature of 37 °C for 5–15 minutes. |
| Storage Conditions | Store at -25 to -15 °C. |
| Shelf Life | 2 years. |
| Package Specifications | 100 T |
| Concentration | 20 U/μL |
| Source / Origin | Recombinant enzyme produced by genetic engineering technology. |
| Expiration Date / Stability | Stable for 2 years when stored at -25 to -15 °C. |
| Recommended Buffer System | All fast restriction enzymes in this series share a single universal fast digestion buffer (1×), which streamlines and simplifies the digestion reaction setup. |
| Application Notes / Precautions | The shared universal buffer simplifies the digestion system; the enzyme offers good activity overcapacity for digesting excess substrate or difficult templates. CpG methylation fully overlapping the recognition sequence blocks cleavage; consider the methylation status of the DNA substrate. Heat-inactivate at 80 °C for 20 min when downstream applications require enzyme removal. |
| Recognition Site | 5'-A↓CGCGT-3' 3'-TGCGC↑A-5' |
| Heat Inactivation | Incubate at 80 °C for 20 min. |
| Methylation Sensitivity | CpG: the site fully overlaps the recognition sequence; cleavage is blocked. |
For research use only, not for clinical use.
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