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| Product Name | Fast Restriction Enzyme HinfI (Recognition Site: 5'-G↓ANTC-3') |
| Catalog No. | IVDRM-0046 |
| Description | This fast restriction enzyme is produced by recombinant genetic engineering and completes precise DNA cleavage within 5-15 minutes, making it ideal for rapid digestion of plasmid DNA, PCR products, and genomic DNA. All enzymes in the fast digestion series share a single universal buffer, which greatly simplifies reaction setup. Generous activity redundancy allows easy handling of excess substrate or difficult templates. |
| Intended Use | For rapid digestion of plasmid DNA, PCR products, genomic DNA, and other DNA substrates in molecular cloning and molecular biology workflows. For research use. |
| Principle / Technology | Recombinant restriction endonuclease produced by genetic engineering. It recognizes a specific double-stranded DNA sequence and cleaves both strands at defined positions, completing accurate digestion within 5-15 minutes. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA, and other DNA substrates. |
| Performance Range / Specifications | Recognition site: 5'-G↓ANTC-3' 3'-CTNA↑G-5' Enzyme activity: 50 U/μL Digestion time: 5-15 min Heat inactivation: 80°C for 20 min |
| Specificity | Specifically recognizes and cleaves the sequence 5'-G↓ANTC-3'. |
| Reaction Conditions / Protocol | Recommended reaction conditions: 1× universal fast digestion buffer; optimal incubation at 37°C for 5-15 min. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 2 years. |
| Package Specifications | 500 T |
| Concentration | 50 U/μL |
| Activity / Unit Definition | Enzyme activity: 50 U/μL. |
| Source / Origin | Recombinant enzyme produced by genetic engineering. |
| Expiration Date / Stability | Stable for 2 years when stored at -25°C to -15°C. |
| Recommended Buffer System | A single universal fast digestion buffer is shared across the entire fast restriction enzyme series, simplifying the digestion reaction setup. |
| Application Notes / Precautions | Good activity redundancy allows easy handling of excess substrate or difficult templates. Methylation note: CpG and EcoBI methylation may overlap the recognition sequence and block cleavage. |
| Recognition Site | 5'-G↓ANTC-3' 3'-CTNA↑G-5' |
| Heat Inactivation | Incubate at 80°C for 20 min. |
| Methylation Sensitivity | CpG, EcoBI: sequence overlap is possible, resulting in blocked cleavage. |
For research use only, not for clinical use.
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