- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Fast Restriction Enzyme HhaI (Recognition Site: 5'-GCG↓C-3') |
| Catalog No. | IVDRM-0049 |
| Description | This fast restriction enzyme is produced by recombinant genetic engineering and completes precise DNA cleavage within 5-15 minutes, making it ideal for rapid digestion of plasmid DNA, PCR products, and genomic DNA. All enzymes in the fast digestion series share a single universal buffer, which greatly simplifies reaction setup. Generous activity redundancy allows easy handling of excess substrate or difficult templates. |
| Intended Use | For rapid digestion of plasmid DNA, PCR products, genomic DNA, and other DNA substrates in molecular cloning and molecular biology workflows. For research use. |
| Principle / Technology | Recombinant restriction endonuclease produced by genetic engineering. It recognizes a specific double-stranded DNA sequence and cleaves both strands at defined positions, completing accurate digestion within 5-15 minutes. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA, and other DNA substrates. |
| Performance Range / Specifications | Recognition site: 5'-GCG↓C-3' 3'-C↑GCG-5' Enzyme activity: 20 U/μL Digestion time: 5-15 min Heat inactivation: 80°C for 20 min |
| Specificity | Specifically recognizes and cleaves the sequence 5'-GCG↓C-3'. |
| Reaction Conditions / Protocol | Recommended reaction conditions: 1× universal fast digestion buffer; optimal incubation at 37°C for 5-15 min. |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 2 years. |
| Package Specifications | 100 T |
| Concentration | 20 U/μL |
| Activity / Unit Definition | Enzyme activity: 20 U/μL. |
| Source / Origin | Recombinant enzyme produced by genetic engineering. |
| Expiration Date / Stability | Stable for 2 years when stored at -25°C to -15°C. |
| Compatibility | Isoschizomers: HinP1I, CfoI, AspLEI, Hin6I, HspAI, BstHHI |
| Recommended Buffer System | A single universal fast digestion buffer is shared across the entire fast restriction enzyme series, simplifying the digestion reaction setup. |
| Application Notes / Precautions | Good activity redundancy allows easy handling of excess substrate or difficult templates. Methylation note: CpG methylation affects cleavage by HhaI; Dam, Dcm, EcoKI, and EcoBI methylation have no effect. |
| Recognition Site | 5'-GCG↓C-3' 3'-C↑GCG-5' |
| Heat Inactivation | Incubate at 80°C for 20 min. |
| Methylation Sensitivity | CpG: cleavage is affected. Dam, Dcm, EcoKI, EcoBI: no effect. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
