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| Product Name | Fast Restriction Enzyme FokI (Recognition Site: GGATG(N)9) |
| Catalog No. | IVDRM-0043 |
| Description | This fast restriction enzyme is produced by recombinant technology and delivers precise DNA cleavage within 5-15 minutes. It is well suited for rapid digestion of plasmid DNA, PCR products, and genomic DNA. The enzyme shares a single universal reaction buffer, which streamlines reaction setup, and its generous activity overage allows it to handle excess substrate or difficult templates with ease. |
| Intended Use | For rapid digestion of plasmid DNA, PCR products, and genomic DNA in molecular biology and molecular cloning workflows. |
| Principle / Technology | Recombinant fast restriction endonuclease engineered by recombinant technology; it recognizes a specific DNA sequence and completes precise cleavage within 5-15 minutes. |
| Sample Type | Plasmid DNA, PCR products, and genomic DNA. |
| Performance Range / Specifications | Recognition and cleavage site: 5'-GGATG(N)9↓-3' 3'-CCTAC(N)13↑-5' Enzyme activity: 5 U/μL Isoschizomers: BstF5I, BtsCI, BseGI Effect of methylation: Dam, CpG, EcoBI: cleavage is blocked. Dcm, EcoKI: no effect. |
| Specificity | Recognizes and cleaves the specific recognition sequence with high fidelity; isoschizomers: BstF5I, BtsCI, BseGI. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; optimal incubation at 37°C. |
| Storage Conditions | Store at -25 to -15°C; valid for 2 years. |
| Shelf Life | 2 years. |
| Package Specifications | 100 T |
| Concentration | 5 U/μL |
| Activity / Unit Definition | Enzyme activity: 5 U/μL. |
| Source / Origin | Produced by recombinant technology. |
| Expiration Date / Stability | Stable for 2 years when stored at the recommended temperature. |
| Compatibility | BstF5I, BtsCI, BseGI |
| Recommended Buffer System | All fast restriction enzymes in this series share a single universal digestion buffer, simplifying the digestion reaction setup. |
| Application Notes / Precautions | Effect of methylation on cleavage: Dam, CpG, EcoBI: cleavage is blocked. Dcm, EcoKI: no effect. Complete digestion is typically achieved within 5-15 minutes; excess activity allows digestion of substrate overage or difficult templates. |
| Recognition Site | 5'-GGATG(N)9↓-3' 3'-CCTAC(N)13↑-5' |
| Heat Inactivation | Incubation at 80°C for 20 min. |
| Methylation Sensitivity | Dam, CpG, EcoBI: cleavage is blocked. Dcm, EcoKI: no effect. |
For research use only, not for clinical use.
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