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| Product Name | Fast Restriction Enzyme DdeI (Recognition Site: C/TNAG) |
| Catalog No. | IVDRM-0034 |
| Description | This fast restriction enzyme DdeI is a recombinant enzyme produced by genetic engineering that completes precise DNA cleavage within 5–15 minutes. It is well suited for rapid digestion of plasmid DNA, PCR products, and genomic DNA. A single universal digestion buffer is shared across the fast enzyme series, greatly simplifying reaction setup. With generous activity redundancy, the enzyme readily handles excess substrate and difficult templates. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products, and genomic DNA in molecular cloning and molecular biology workflows. |
| Principle / Technology | DdeI is a type II restriction endonuclease that recognizes the palindromic sequence 5'-C/TNAG-3' and cleaves DNA at defined positions, generating fragments with compatible termini for downstream cloning and analysis. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA. |
| Performance Range / Specifications | Complete digestion within 5–15 minutes; good activity redundancy allows digestion of excess substrate or difficult templates. |
| Specificity | Recognizes and cleaves at 5'-C↓TNAG-3' / 3'-GANT↑C-5'. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; incubate at 37°C. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years when stored at -25 to -15°C. |
| Package Specifications | 50 T |
| Concentration | 10 U/μL |
| Activity / Unit Definition | 10 U/μL |
| Source / Origin | Recombinant enzyme produced by genetic engineering. |
| Expiration Date / Stability | Stable for 2 years at -25 to -15°C. |
| Recommended Buffer System | 1× universal fast digestion buffer shared across the fast restriction enzyme series, simplifying multi-enzyme reaction setup. |
| Recognition Site | 5'-C↓TNAG-3' 3'-GANT↑C-5' |
| Heat Inactivation | 80°C for 20 min. |
| Methylation Sensitivity | EcoBI: cleavage blocked Dam, Dcm, CpG, EcoKI: no effect |
For research use only, not for clinical use.
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