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| Product Name | Fast Restriction Enzyme Cfr42I (SacII) (Recognition Site: 5'-CCGC↓GG-3') |
| Catalog No. | IVDRM-0029 |
| Description | Fast Restriction Enzyme Cfr42I (SacII) is a fast restriction endonuclease produced by recombinant genetic engineering. It precisely completes DNA cleavage within 5–15 minutes and is suitable for rapid digestion of plasmid DNA, PCR products, genomic DNA, and more. All fast restriction enzymes in this series share a single universal digestion buffer, which greatly simplifies the reaction setup. In addition, the enzyme provides ample activity redundancy, allowing effortless digestion of excess substrate or difficult templates. Cfr42I (SacII) recognizes the sequence 5'-CCGC↓GG-3' and cleaves to produce the corresponding cohesive ends. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products, and genomic DNA in 5–15 minutes for molecular cloning and other molecular biology workflows. |
| Principle / Technology | Recombinant fast restriction endonuclease that recognizes a specific double-stranded DNA sequence and cleaves it within 5–15 minutes; a single universal fast digestion buffer is shared across the series to simplify reaction setup, with generous activity redundancy to handle excess substrate or difficult templates. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA. |
| Performance Range / Specifications | Recognition site: 5'-CCGC↓GG-3' (3'-GG↑CGCC-5') Enzyme activity: 20 U/μL Heat inactivation: 80°C for 20 min |
| Specificity | Recognizes and cleaves 5'-CCGC↓GG-3' / 3'-GG↑CGCC-5'. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; optimal incubation at 37°C. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years when stored at -25 to -15°C. |
| Package Specifications | 60 T |
| Concentration | 20 U/μL |
| Expiration Date / Stability | Stable for 2 years at -25 to -15°C. |
| Compatibility | Isoschizomers: SacII, KspI, Sfr303I, SgrBI |
| Recommended Buffer System | A single universal fast digestion buffer is shared across the fast restriction enzyme series, simplifying reaction setup (use at 1×). |
| Application Notes / Precautions | CpG methylation may block cleavage. |
| Recognition Site | 5'-CCGC↓GG-3' 3'-GG↑CGCC-5' |
| Heat Inactivation | Incubate at 80°C for 20 min. |
| Methylation Sensitivity | CpG: cleavage may be blocked. |
For research use only, not for clinical use.
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