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| Product Name | Fast Restriction Enzyme BglI (Recognition Site: GCC(N)4↓NGGC) |
| Catalog No. | IVDRM-0023 |
| Description | This fast restriction enzyme, produced by recombinant genetic engineering, completes precise DNA cleavage within 5-15 minutes and is ideal for rapid digestion of plasmid DNA, PCR products, and genomic DNA. All enzymes in the series share a single universal digestion buffer, which simplifies reaction setup, and provide a generous activity surplus, enabling effortless digestion of excess substrate or difficult templates. |
| Intended Use | For rapid digestion of plasmid DNA, PCR products, and genomic DNA in molecular cloning and related molecular biology workflows. |
| Principle / Technology | Recombinant restriction endonuclease produced by genetic engineering; it specifically recognizes and cleaves double-stranded DNA at its defined recognition sequence. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA. |
| Performance Range / Specifications | Completes precise DNA digestion in 5-15 min; provides ample activity surplus to handle excess substrate or difficult templates. |
| Specificity | Specifically recognizes and cleaves the sequence 5'-GCC(N)4↓NGGC-3' 3'-CGGN↑(N)4CCG-5'. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; incubate at 37°C. |
| Storage Conditions | Store at -25 to -15°C. |
| Shelf Life | 2 years when stored at -25 to -15°C. |
| Package Specifications | 200 T |
| Concentration | 10 U/μL |
| Source / Origin | Recombinant enzyme produced by genetic engineering. |
| Expiration Date / Stability | Stable for 2 years when stored at -25 to -15°C. |
| Recommended Buffer System | Shares a single universal fast digestion buffer with other enzymes in the series, simplifying the digestion reaction setup. |
| Application Notes / Precautions | Use 1× universal fast digestion buffer and incubate at 37°C; complete digestion is typically achieved within 5-15 min. The enzyme offers sufficient activity surplus for digestion of excess substrate or difficult templates. |
| Recognition Site | 5'-GCC(N)4↓NGGC-3' 3'-CGGN↑(N)4CCG-5' |
| Heat Inactivation | Incubate at 80°C for 20 min. |
| Methylation Sensitivity | CpG: cleavage affected Dam, Dcm, EcoKI, EcoBI: no effect |
For research use only, not for clinical use.
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