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| Product Name | Fast Restriction Enzyme BamHI (Recognition Site: G/GATCC) |
| Catalog No. | IVDRM-0015 |
| Description | Fast Restriction Enzyme BamHI from Alta DiagnoTech is a recombinant rapid restriction endonuclease that completes precise DNA cleavage within 5-15 minutes. It is well suited for rapid digestion of plasmid DNA, PCR products and genomic DNA. All enzymes in the fast digestion series share one universal buffer, which simplifies reaction setup, and their excellent activity redundancy easily handles excess substrate or difficult templates. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA for molecular cloning and related molecular biology workflows. |
| Principle / Technology | Recombinant fast restriction endonuclease produced by genetic engineering; recognizes the specific double-stranded DNA sequence 5'-G/GATCC-3' and cleaves rapidly at 37°C in a universal fast digestion buffer. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Complete digestion within 5-15 min Supplied at 20 U/μL Good activity redundancy - readily digests excess substrate or difficult templates |
| Specificity | Specifically recognizes 5'-G/GATCC-3'; cleavage is unaffected by Dam, Dcm, CpG, EcoKI or EcoBI methylation. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; optimal incubation at 37°C |
| Storage Conditions | -25°C to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 500 T |
| Concentration | 20 U/μL |
| Source / Origin | Recombinant (produced by genetic engineering technology) |
| Expiration Date / Stability | Stable for 2 years when stored at -25°C to -15°C. |
| Recommended Buffer System | A single universal fast digestion buffer is shared across the entire fast restriction enzyme series, simplifying single- and multi-enzyme digestion setups. |
| Application Notes / Precautions | This enzyme cannot be heat-inactivated; remove it by phenol-chloroform extraction or column purification before downstream applications. Cleavage is not affected by Dam, Dcm, CpG, EcoKI or EcoBI methylation. |
| Recognition Site | 5'-G↓GATCC-3' 3'-CCTAG↑G-5' |
| Heat Inactivation | Not heat-inactivatable; use phenol-chloroform extraction or column purification |
| Methylation Sensitivity | Dam, Dcm, CpG, EcoKI, EcoBI: no effect |
For research use only, not for clinical use.
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