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| Product Name | Fast Restriction Enzyme AvaII (Recognition Site: G/GWCC) |
| Catalog No. | IVDRM-0011 |
| Description | Fast Restriction Enzyme AvaII from Alta DiagnoTech is a recombinant rapid restriction endonuclease engineered for fast, accurate DNA cleavage. It completes digestion within 5-15 minutes and is well suited for plasmid DNA, PCR products, genomic DNA and other common substrates. All enzymes in the fast digestion series share a single universal buffer, which greatly simplifies reaction setup. A generous activity redundancy allows the enzyme to handle excess substrate or difficult templates with ease. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA for molecular cloning and related molecular biology workflows. |
| Principle / Technology | Recombinant fast restriction endonuclease produced by genetic engineering; recognizes the specific double-stranded DNA sequence 5'-G/GWCC-3' and cleaves rapidly at 37°C in a universal fast digestion buffer. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Complete digestion within 5-15 min Supplied at 10 U/μL Good activity redundancy - readily digests excess substrate or difficult templates |
| Specificity | Specifically recognizes 5'-G/GWCC-3'; CpG and Dcm methylation may block cleavage. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; incubate at 37°C |
| Storage Conditions | -25°C to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Concentration | 10 U/μL |
| Source / Origin | Recombinant (produced by genetic engineering technology) |
| Expiration Date / Stability | Stable for 2 years when stored at -25°C to -15°C. |
| Compatibility | Isoschizomers: Bme18I, Eco47I |
| Recommended Buffer System | A single universal fast digestion buffer is shared across the entire fast restriction enzyme series, simplifying single- and multi-enzyme digestion setups. |
| Application Notes / Precautions | CpG and Dcm methylation of the recognition site may block cleavage; verify the methylation status of the substrate DNA when digesting mammalian or Dam/Dcm-positive DNA. |
| Recognition Site | 5'-G↓GWCC-3' 3'-CCWG↑G-5' |
| Heat Inactivation | 80°C for 20 min |
| Methylation Sensitivity | CpG, Dcm: cleavage may be blocked |
For research use only, not for clinical use.
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