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| Product Name | Fast Restriction Enzyme ApaLI (Recognition Site: G/TGCAC) |
| Catalog No. | IVDRM-0003 |
| Description | ApaLI is a recombinant fast restriction endonuclease engineered to deliver precise, complete DNA cleavage within just 5–15 minutes. It is ideally suited for the rapid digestion of plasmid DNA, PCR products, and genomic DNA. All enzymes in this fast-digestion series share a single universal digestion buffer, greatly simplifying reaction setup, and provide generous activity redundancy to effortlessly handle excess substrate or difficult templates. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products, and genomic DNA for molecular cloning workflows |
| Principle / Technology | Recombinant fast restriction endonuclease produced by genetic engineering (recombinant expression) technology, enabling precise and complete DNA cleavage within 5-15 min |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Precise, complete DNA digestion within 5-15 min; high activity redundancy allows digestion of excess substrate or difficult templates |
| Specificity | Site-specific endonuclease recognizing G↓TGCAC; CpG methylation: sequence may overlap, cleavage blocked |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; optimal incubation at 37°C |
| Storage Conditions | -25°C to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 200 T |
| Concentration | 10 U/μL |
| Source / Origin | Recombinant (produced by genetic engineering / recombinant expression technology) |
| Expiration Date / Stability | Stable for 2 years when stored at -25°C to -15°C |
| Recommended Buffer System | All enzymes in this fast-digestion series share one universal digestion buffer, simplifying the reaction setup |
| Application Notes / Precautions | Note: CpG methylation: sequence may overlap, cleavage blocked. |
| Recognition Site | 5'-G↓TGCAC-3' 3'-CACGT↑G-5' |
| Heat Inactivation | 80°C for 20 min |
| Methylation Sensitivity | CpG: sequence may overlap, cleavage blocked |
For research use only, not for clinical use.
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