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| Product Name | Fast Restriction Enzyme AluI (Recognition Site: AG/CT) |
| Catalog No. | IVDRM-0006 |
| Description | This enzyme is a fast restriction endonuclease produced by recombinant genetic engineering. It delivers precise DNA cleavage within 5-15 minutes and is well suited for rapid digestion of plasmid DNA, PCR products, and genomic DNA. All enzymes in this fast digestion series share one universal buffer, which greatly simplifies the reaction setup, and the enzyme provides generous activity redundancy, allowing effortless digestion of excess substrate or difficult templates. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products, and genomic DNA |
| Principle / Technology | Recombinant fast restriction endonuclease produced by genetic engineering technology; cleaves DNA rapidly and precisely at its specific recognition sequence. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Complete and precise DNA digestion within 5-15 minutes Good activity redundancy - easily handles excess substrate or difficult templates |
| Specificity | Specifically recognizes and cleaves 5'-AG↓CT-3'. CpG and EcoBI methylation blocks cleavage; Dam, Dcm and EcoKI methylation has no effect. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; incubate at 37°C |
| Storage Conditions | Store at -25°C to -15°C. |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Concentration | 10 U/μL |
| Source / Origin | Recombinant (produced by genetic engineering / recombinant expression technology) |
| Expiration Date / Stability | Stable for 2 years when stored at -25°C to -15°C. |
| Compatibility | AluBI |
| Recommended Buffer System | A single universal fast digestion buffer is shared across the fast restriction enzyme series, simplifying the reaction setup for single, double, or multiple digests. |
| Application Notes / Precautions | CpG or EcoBI methylation at the recognition site blocks cleavage; Dam, Dcm and EcoKI methylation does not affect the reaction. The enzyme can be heat-inactivated at 80°C for 20 min. |
| Recognition Site | 5'-AG↓CT-3' 3'-TC↑GA-5' |
| Heat Inactivation | 80°C for 20 min |
| Methylation Sensitivity | CpG, EcoBI: cleavage blocked Dam, Dcm, EcoKI: no effect |
For research use only, not for clinical use.
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