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| Product Name | Fast Restriction Enzyme AatII (Recognition Site: GACGT/C) |
| Catalog No. | IVDRM-0012 |
| Description | Fast Restriction Enzyme AatII from Alta DiagnoTech is a recombinant rapid restriction endonuclease that performs precise DNA cleavage within 5-15 minutes. It is ideal for quick digestion of plasmid DNA, PCR products and genomic DNA. Every enzyme in the fast digestion series works in the same universal buffer, streamlining reaction setup, and its high activity redundancy makes it easy to digest excess substrate or difficult templates. |
| Intended Use | Rapid digestion of plasmid DNA, PCR products and genomic DNA for molecular cloning and related molecular biology workflows. |
| Principle / Technology | Recombinant fast restriction endonuclease produced by genetic engineering; recognizes the specific double-stranded DNA sequence 5'-GACGT/C-3' and cleaves rapidly at 37°C in a universal fast digestion buffer. |
| Sample Type | Plasmid DNA, PCR products, genomic DNA |
| Performance Range / Specifications | Complete digestion within 5-15 min Supplied at 20 U/μL Good activity redundancy - readily digests excess substrate or difficult templates |
| Specificity | Specifically recognizes 5'-GACGT/C-3'; CpG methylation may block cleavage. |
| Reaction Conditions / Protocol | 1× universal fast digestion buffer; incubate at 37°C |
| Storage Conditions | -25°C to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 30 T |
| Concentration | 20 U/μL |
| Source / Origin | Recombinant (produced by genetic engineering technology) |
| Expiration Date / Stability | Stable for 2 years when stored at -25°C to -15°C. |
| Compatibility | Isoschizomers: ZraI |
| Recommended Buffer System | A single universal fast digestion buffer is shared across the entire fast restriction enzyme series, simplifying single- and multi-enzyme digestion setups. |
| Application Notes / Precautions | CpG methylation of the recognition site may block cleavage; check the methylation status of the substrate DNA when working with CpG-methylated templates. |
| Recognition Site | 5'-GACGT↓C-3' 3'-C↑TGCAG-5' |
| Heat Inactivation | 80°C for 20 min |
| Methylation Sensitivity | CpG: cleavage may be blocked |
For research use only, not for clinical use.
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