- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Fast PNGase F, Non-Reducing Format (Rapid N-Deglycosylation, 20 T / 50 T) |
| Catalog No. | IVDRM-0294 |
| Description | Conventional peptide-N-glycosidase takes hours to release antibody glycans, and because some species are released faster than others, a partial reaction distorts the apparent glycan profile. That error matters when glycan distribution is a quality attribute of a therapeutic antibody. This preparation is engineered for speed, finishing the digestion in minutes, and it is formulated without reducing agents so the disulphide pattern and higher-order structure survive. Keeping the protein intact is what makes it useful for analysis of the whole molecule by mass spectrometry, and for high-throughput proteomics where a short, uniform step is worth more than absolute completeness at longer incubation. |
| Intended Use | Rapid removal of N-linked glycans from antibodies, immunoglobulins, fusion proteins and other glycoproteins ahead of intact protein mass spectrometry and high-throughput proteomic characterisation. |
| Principle / Technology | The amidase cleaves the amide bond between the asparagine-linked innermost sugar of the glycan and the peptide backbone; without reducing agent and with short incubation the protein disulphide framework stays linked together. |
| Detection Method | Released glycans and deglycosylated protein are analysed by mass spectrometry or chromatography rather than by a signal from the enzyme. |
| Sample Type | Purified antibodies, fusion proteins and glycoproteins in solution. |
| Performance Range / Specifications | Deglycosylation completed within minutes instead of hours; non-reducing conditions preserve disulphide bonds; retains higher-order structure; compatible with intact-protein mass spectrometry workflows. |
| Specificity | Supplied with a 5x concentrated reaction buffer; 20-test vial contains 20 uL of enzyme and 80 uL of buffer; 50-test vial contains 50 uL and 200 uL respectively; denaturation at 75 °C for 5 minutes followed by digestion at 50 °C for 10 minutes. |
| Reaction Conditions / Protocol | Denature the sample for 5 minutes at 75 °C with the supplied buffer, cool, add the enzyme and incubate for about 10 minutes at 50 °C, then move the digest directly into mass spectrometry or chromatographic analysis. |
| Components / Formulation | Enzyme solution and 5x reaction buffer in matched volumes. |
| Storage Conditions | Store at 2 to 8 °C; never freeze. |
| Shelf Life | 1 year from receipt when refrigerated. |
| Package Specifications | 20-test and 50-test presentations, each with enzyme plus 5x buffer. |
| Product Form | Clear enzyme solution with separate concentrated buffer. |
| Quality Control | Each lot is checked for digestion completeness against a reference antibody substrate. |
| Key Features | - Completes deglycosylation in minutes rather than hours - Non-reducing formulation keeps disulphide bonds intact - Higher-order structure is preserved for whole-molecule mass analysis - Supplied with matching 5x reaction buffer - Suited to high-throughput proteomics workflows |
| Purity | Recombinant enzyme purified to remove contaminating glycosidase activity. |
| Concentration | Supplied ready to use with the companion buffer; see the test count for reaction numbers. |
| Activity / Unit Definition | Defined by complete glycan release from a reference glycoprotein within the stated short incubation rather than by standard units. |
| Molecular Weight | Not separately stated on the source page for this format. |
| Source / Origin | Recombinantly expressed in yeast. |
| pH Range / Optimal pH | Reaction buffer is 20 mM Tris-HCl with 50 mM sodium chloride and 5 mM EDTA at pH 7.5. |
| Shipping Conditions | Shipped cold, kept chilled rather than frozen. |
| Expiration Date / Stability | Stability depends on continuous storage at the stated temperature with the container closed. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Digests feed directly into chromatographic or mass spectrometric analysis without a desalting requirement. |
| Recommended Buffer System | 20 mM Tris-HCl, 50 mM sodium chloride, 5 mM EDTA at pH 7.5 supplied as a 5x concentrate. |
| Application Notes / Precautions | Do not freeze the enzyme; keep it refrigerated; work under the non-reducing protocol so the quaternary structure of the substrate remains intact. |
| Batch-to-Batch Consistency | Fixed production controls and release testing keep performance uniform from lot to lot. |
| Model Type | Rapid non-reducing N-deglycosylation reagent |
| Starting Material / Tissue Site | Yeast-expressed recombinant amidase |
| Delivery Standard | Enzyme vial plus buffer vial |
| Readouts / Downstream Assays | Mass shift of the intact protein after glycan release and the glycan profile obtained downstream. |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
