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| Product Name | Fast-Growth T1/T5-Resistant Chemically Competent Cells |
| Catalog No. | ATR-CCR-0044 |
| Description | Fast-growing chemically competent cloning cells developed for rapid transformation and accelerated colony recovery. |
| Intended Use | Rapid transformation of ampicillin-resistant plasmids and ligation products; routine plasmid cloning and propagation. |
| Principle / Technology | Chemically competent cells take up exogenous plasmid DNA after a standard transformation treatment and recover rapidly on selective medium. |
| Sample Type | Plasmid DNA and ligation products. |
| Performance Range / Specifications | Colonies become visible after 8–9 hours on ampicillin plates. Overnight single colonies grown in 2 mL LB can be used for miniprep after 4–5 hours of culture. A five-minute rapid transformation workflow is supported for ampicillin-resistant plasmids and ligation products. |
| Reaction Conditions / Protocol | For compatible ampicillin-resistant plasmids or ligation products, use the rapid five-minute transformation workflow described in the protocol. |
| Components / Formulation | Aliquoted chemically competent Escherichia coli cell suspension. |
| Storage Conditions | Store at −70°C. Do not store at −20°C or in liquid nitrogen. |
| Package Specifications | 10 × 100 µL; 20 × 100 µL. |
| Product Form | Chemically competent E. coli cells. |
| Key Features | Fast colony growth; rapid transformation; T1 and T5 phage resistance; suitable for routine cloning workflows. |
| Compatibility | Compatible with ampicillin-resistant plasmids and ligation products. |
| Application Notes / Precautions | Keep the cells frozen until use and avoid repeated freeze–thaw cycles. |
For research use only, not for clinical use.
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