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| Product Name | Extreme Thermostable Single-Strand DNA Binding Protein, ET SSB (0.5 mg/mL) |
| Catalog No. | IVDRM-0512 |
| Description | A sequence-independent single-stranded DNA binding protein from an extreme thermophile that binds ssDNA but not RNA or double stranded DNA. It coats the single-stranded regions generated as helicases and polymerases progress, preventing the newly formed single strands from re-pairing or from being digested by nucleases, and upon binding it destabilises secondary structure so that DNA polymerases reach their substrate more easily. This raises polymerase catalytic activity and improves amplification efficiency in PCR on complex templates, and it also stabilises single stranded DNA in high temperature sequencing reactions. The protein keeps its full activity after 60 minutes at 95°C, works in most polymerase buffers and is free of exonuclease, nickase and RNase residue. |
| Intended Use | PCR additive for complex templates, high temperature DNA sequencing and protection of single stranded DNA from nuclease digestion |
| Principle / Technology | Sequence-independent coating of single-stranded DNA that prevents re-pairing and nuclease digestion and stimulates polymerase activity |
| Sample Type | Single stranded DNA regions in PCR and sequencing reactions |
| Specificity | Binds single-stranded DNA only, not RNA or double stranded DNA; full activity retained after 60 minutes at 95°C |
| Reaction Conditions / Protocol | Add to the PCR or sequencing reaction mix at the recommended amount for template stabilisation |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 ug; 200 ug |
| Key Features | Extremely thermostable; active in most polymerase buffers; host genomic DNA below 1 copy per 2.5 ug |
| Purity | Greater than or equal to 95%; free of exonuclease, nickase and RNase residue |
| Concentration | 0.5 mg/mL |
| Readouts / Downstream Assays | Agarose gel electrophoresis of amplified products |
For in vitro diagnostic use.
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