- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Exosome Serum-Free Medium (for MSC) |
| Catalog No. | RESK-0617 |
| Description | Exosomes carry important cargos such as proteins, lipids, DNA and RNA, play important roles in intercellular material and information transfer, and are promising biomarkers for the early diagnosis of many diseases. Mesenchymal stem cells (MSCs) can regulate immune cell activity and stimulate tissue regeneration. Exosomes secreted by MSCs through paracrine mechanisms contain characteristic protein markers such as TSG101, CD9, CD63 and CD81, and the bioactive substances they carry, including proteins, lipids, microRNAs and long non-coding RNAs, mediate the therapeutic effects of MSCs and deliver functional genetic information. MSCs are currently recognized as the cells with the strongest exosome-secreting capacity; they can regulate exosome release through specific signaling axes, and changes in the extracellular environment can also affect the exosome-secreting ability and activity of MSCs. This exosome serum-free medium dedicated to MSCs is a medium system suitable for MSC cells, specifically designed for the preparation and production of exosomes from these cells. No serum or platelet lysate needs to be added during culture, which reduces interference from exogenous particles and promotes exosome secretion by MSCs. The product is suitable for exosome production from various MSCs, but cannot be used for MSC passaging. |
| Intended Use | Serum-free culture of mesenchymal stem cells (MSCs) for the preparation and production of MSC-derived exosomes. |
| Principle / Technology | A chemically defined, serum-free medium system that supports MSC culture without adding serum or platelet lysate, reducing interference from exogenous particles and promoting exosome secretion by MSCs. |
| Sample Type | Mesenchymal stem cells (MSCs). |
| Performance Range / Specifications | Serum-free and chemically defined. Contains phenol red, glutamine and sodium bicarbonate. Free of xenogeneic components; pharmaceutical-grade proteins and factors are used. |
| Specificity | Suitable for exosome production from various MSCs; not for MSC passaging. |
| Reaction Conditions / Protocol | 1) Culture MSCs in a conventional medium; when adherent confluence reaches 25-50%, remove the medium. 2) Wash the cells 3-5 times with PBS to avoid contamination by exogenous impurities. 3) Mix the exosome-dedicated MSC basal medium (Component A) with the supplement (Component B) at a ratio of 100:1 to prepare the complete medium; no additional glutamine is required. Reference volumes of complete medium: 3 mL per well of a 6-well plate, 5 mL per 60 mm dish, 10 mL per 100 mm dish, 15 mL per 150 mm dish. 4) Culture MSCs in the complete medium for 24-48 h; when cell confluence reaches about 80%, collect the supernatant for exosome extraction. To collect more exosomes, the medium can be replaced and supernatant collected every 24 h while the cells remain in good condition. |
| Components / Formulation | Component A: exosome-dedicated MSC basal medium Component B: supplement |
| Storage Conditions | Component A at 2-8 °C; Component B at -25 to -15 °C. |
| Shelf Life | 1 year |
| Package Specifications | 50 mL, 500 mL |
| Product Form | Liquid medium (two-component system) |
| Expiration Date / Stability | Shelf life 1 year when components are stored as recommended. Prepare the complete medium fresh before use; store at 2-8 °C and use within one week. For multiple preparations, aliquot the supplement (Component B) and store frozen. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable for exosome production from various mesenchymal stem cells; not for MSC passaging. |
| Recommended Buffer System | Wash cells with PBS before switching to the complete medium. |
| Application Notes / Precautions | No serum or platelet lysate needs to be added during culture. The complete medium should be prepared fresh before use, stored at 2-8 °C and used within one week. If the complete medium is prepared multiple times, aliquot the supplement (Component B) and store it frozen. The product cannot be used for MSC passaging. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
