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| Product Name | Exosome-Depleted Fetal Bovine Serum |
| Catalog No. | RESK-0274 |
| Description | Exosomes are small vesicles of approximately 30-150 nm in diameter secreted by living cells, with a typical lipid bilayer structure. They are found in cell culture supernatants, serum, plasma, saliva, urine, amniotic fluid, and other biological fluids. Exosomes carry important information such as proteins, lipids, DNA, and RNA, playing a key role in intercellular material and information transfer, and are expected to become early diagnostic biomarkers for a variety of diseases. This product is fetal bovine serum collected aseptically from healthy fetal bovines. After purification, >=97% of endogenous exosomes have been removed. It is sterilized by three rounds of 0.1 um filtration, requires no further filtration, and shows minimal batch-to-batch variation. It is suitable for in vitro cell proliferation culture and for studies harvesting exosomes secreted by target cells. |
| Intended Use | In vitro cell proliferation culture; studies involving the harvest of exosomes secreted by target cells (exosome research). For research use only. |
| Principle / Technology | Healthy fetal bovine serum is aseptically collected and purified to remove >=97% of endogenous exosomes, then sterilized by three rounds of 0.1 um filtration, providing a serum supplement that does not introduce background exosomes into exosome research workflows. |
| Sample Type | Cell culture supplement for mammalian cells (e.g., 293T, RAW 264.7, HepG2). |
| Performance Range / Specifications | English synonym: Exosome-depleted Fetal Bovine Serum Endogenous exosome removal: >=97% Endotoxin level: <3 EU/mL Hemoglobin content: 134 mg/L Protein content: 36 g/L Mycoplasma testing: negative E. coli bacteriophage: negative Endogenous exosome detection: 8.9 x 10^9 particles/mL Minimal batch-to-batch variation Cell passage performance verified in 293T, RAW 264.7, and HepG2 cells (comparable growth to control serum through passage 6) |
| Reaction Conditions / Protocol | Thawing: After removal from low-temperature storage, place the serum at 2-8 °C for 12-24 h to partially thaw, then allow it to thaw completely at room temperature. Shake regularly and evenly during thawing. Aliquoting: If a whole bottle cannot be used at once, aliquot aseptically and store frozen to avoid repeated freeze-thaw cycles. |
| Components / Formulation | Aseptically collected healthy fetal bovine serum, purified to remove >=97% of endogenous exosomes; triple-filtered through 0.1 um filters. |
| Storage Conditions | Store frozen at -20 °C or below; avoid repeated freeze-thaw cycles. |
| Shelf Life | 5 years when stored frozen at -20 °C or below. |
| Package Specifications | 50 mL |
| Product Form | Frozen liquid serum, sterile-filtered (3x 0.1 um). |
| Quality Control | Endotoxin level: <3 EU/mL Hemoglobin content: 134 mg/L Protein content: 36 g/L Mycoplasma testing: negative E. coli bacteriophage: negative Endogenous exosome detection: 8.9 x 10^9 particles/mL Endogenous exosome removal: >=97% |
| Source / Origin | Healthy fetal bovine serum, aseptically collected. |
| Expiration Date / Stability | Shelf life of 5 years when stored at -20 °C or below; avoid repeated freeze-thaw cycles. |
| Compatibility | Suitable for in vitro proliferation culture of a broad range of mammalian cells, including 293T, RAW 264.7, and HepG2 cells, and compatible with exosome secretion and isolation workflows. |
| Application Notes / Precautions | Thaw gradually: 12-24 h at 2-8 °C, then at room temperature, shaking regularly during thawing. Aliquot aseptically if a bottle cannot be used at once; avoid repeated freeze-thaw cycles. Do not use if: 1) the packaging is damaged; 2) the solution is turbid or contamination is suspected; 3) the product has exceeded its stated shelf life. No additional filtration is required before use. |
| Batch-to-Batch Consistency | Minimal batch-to-batch variation. |
For research use only, not for clinical use.
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