- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Exonuclease I for Primer Removal and Enzymatic PCR Cleanup (20 U/uL) |
| Catalog No. | IVDRM-0448 |
| Description | Recombinantly expressed from the Escherichia coli Exo I gene, this exonuclease hydrolyses single stranded DNA in the 3 prime to 5 prime direction, stepwise releasing 5 prime monophosphate deoxyribonucleotides and leaving an intact 5 prime terminal dinucleotide. It is highly selective for denatured or single stranded DNA and shows no activity on double stranded DNA, RNA, or strands whose 3 prime hydroxyl is blocked by phosphoryl or acetyl groups. Routine uses include degrading primers after PCR amplification, removing single stranded primers before Sanger sequencing or SNP analysis, cleaning up nested PCR reactions, enzymatic purification of PCR products and stripping 3 prime hydroxyl single stranded DNA from nucleic acid mixtures. |
| Intended Use | Primer degradation after PCR, cleanup before Sanger sequencing and SNP analysis, nested PCR cleanup and enzymatic PCR purification |
| Principle / Technology | 3 prime to 5 prime exonucleolytic digestion of single stranded DNA |
| Detection Method | Single stranded DNA with free 3 prime hydroxyl ends |
| Sample Type | PCR reactions; nucleic acid mixtures |
| Specificity | No activity on double stranded DNA, RNA or 3 prime blocked strands; free of endonuclease and RNase residues |
| Reaction Conditions / Protocol | Digest at 37°C; heat inactivate at 80°C for 15 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1000 U; 5000 U |
| Key Features | Source gene recombinantly expressed in E. coli; pairs with alkaline phosphatase in standard PCR cleanup workflows |
| Readouts / Downstream Assays | Agarose or polyacrylamide gel confirming primer removal |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
