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| Product Name | Enzyme-Free Cell Dissociation Solution, Sterile |
| Catalog No. | RERE-0196 |
| Description | Sterile dissociation solution for cultured cells, also used for the gentle disruption of some tissue samples. The formulation contains no trypsin or other proteolytic enzyme, so cell-surface proteins, including receptors and epitopes used in flow cytometry, are preserved during detachment. This non-enzymatic mechanism is gentler on monolayers than trypsinisation and suits sensitive lines, clonal recovery and workflows where downstream surface staining follows immediately. Supplied sterile; shelf life is 12 months at room temperature. |
| Intended Use | Gentle detachment of adherent cultured cells and dissociation of selected tissue samples where protease exposure is undesirable |
| Principle / Technology | Non-enzymatic formulation loosens cell attachment without proteolysis, keeping surface proteins intact |
| Specificity | Sterile solution; trypsin-free formulation; reduced cell stress relative to enzymatic dissociation; 12 month shelf life |
| Reaction Conditions / Protocol | Remove culture medium, rinse the monolayer with a balanced salt wash, add dissociation solution to cover and incubate until cells round up, then disperse by pipetting and dilute or neutralise as the workflow requires |
| Storage Conditions | RT |
| Package Specifications | 100 mL |
| Key Features | Trypsin-free and sterile; preserves surface epitopes; 12 month shelf life |
For research use only, not for clinical use.
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