Enzyme-Free Cell Dissociation Solution, Sterile
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Enzyme-Free Cell Dissociation Solution, Sterile

Cat.No: RERE-0196 Datasheet

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Product Name Enzyme-Free Cell Dissociation Solution, Sterile
Catalog No. RERE-0196
Description Sterile dissociation solution for cultured cells, also used for the gentle disruption of some tissue samples. The formulation contains no trypsin or other proteolytic enzyme, so cell-surface proteins, including receptors and epitopes used in flow cytometry, are preserved during detachment. This non-enzymatic mechanism is gentler on monolayers than trypsinisation and suits sensitive lines, clonal recovery and workflows where downstream surface staining follows immediately. Supplied sterile; shelf life is 12 months at room temperature.
Intended Use Gentle detachment of adherent cultured cells and dissociation of selected tissue samples where protease exposure is undesirable
Principle / Technology Non-enzymatic formulation loosens cell attachment without proteolysis, keeping surface proteins intact
Specificity Sterile solution; trypsin-free formulation; reduced cell stress relative to enzymatic dissociation; 12 month shelf life
Reaction Conditions / Protocol Remove culture medium, rinse the monolayer with a balanced salt wash, add dissociation solution to cover and incubate until cells round up, then disperse by pipetting and dilute or neutralise as the workflow requires
Storage Conditions RT
Package Specifications 100 mL
Key Features Trypsin-free and sterile; preserves surface epitopes; 12 month shelf life

For research use only, not for clinical use.

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