Endonuclease VIII (10 U/uL)

Name: Endonuclease VIII (10 U/uL)
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Cat.No: IVDRM-0496 Datasheet

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Product Name	Endonuclease VIII (10 U/uL)
Catalog No.	IVDRM-0496
Description	A DNA repair enzyme carrying both N-glycosylase and AP lyase activities, Endonuclease VIII releases damaged pyrimidine bases from duplex DNA through its glycosylase action to create an AP site, and its lyase activity then cuts the phosphodiester bonds on both sides of that site to leave a gap bounded by 5 prime and 3 prime phosphates. Lesions recognised include urea, 5,6-dihydroxythymine, thymine glycol, 5-hydroxy-5-methylhydantoin, uracil glycol, 6-hydroxy-5,6-dihydrothymine and methyltartronylurea. It supports single cell gel electrophoresis, DNA damage repair in NGS library preparation, strand release during enzymatic DNA synthesis, alkaline elution, and cloning of DNA fragments carrying uracil together with UDG. The high purity preparation is free of exonuclease, endonuclease and RNase residue.
Intended Use	Detection of DNA damage, NGS library repair, alkaline elution and uracil-containing fragment cloning
Principle / Technology	N-glycosylase release of damaged pyrimidines followed by AP lyase cleavage on both sides of the AP site
Sample Type	Damaged double stranded DNA
Specificity	Releases urea, dihydroxythymine, thymine glycol, hydroxymethylhydantoin, uracil glycol and related oxidised pyrimidine lesions
Reaction Conditions / Protocol	Incubate with the damaged DNA substrate, then heat at 75°C for 10 minutes to inactivate
Storage Conditions	-25 to -15°C
Shelf Life	1 year
Package Specifications	1000 U; 5000 U
Key Features	Dual glycosylase and AP lyase activity; broad damaged pyrimidine spectrum
Purity	High purity, free of exonuclease, endonuclease and RNase residue
Concentration	10 U/uL
Readouts / Downstream Assays	Agarose gel electrophoresis of cleavage products

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For in vitro diagnostic use.