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| Product Name | Endonuclease V for DNA Repair Research (10 U/uL) |
| Catalog No. | IVDRM-0569 |
| Description | Encoded by the Endo V gene of E. coli, Endonuclease V is a repair enzyme that recognises deoxyinosine in DNA, the deamination product of deoxyadenosine. Also called deoxyinosine 3 prime endonuclease, it cuts the second phosphodiester bond on the 3 prime side of mismatched deoxyinosine to leave a nick carrying a 3 prime hydroxyl and a 5 prime phosphate. The enzyme recognises deoxyinosine whether paired or not in both double and single stranded DNA, and it also recognises DNA containing AP sites, urea residues, base mismatches, insertion and deletion mismatches, hairpins, unpaired loops, folded flaps and Y-shaped structures. In DNA repair the enzyme acts alongside other proteins because it cannot itself remove deoxyinosine or damaged bases from the DNA. |
| Intended Use | Research into repair of DNA deamination damage and structured DNA lesions |
| Principle / Technology | Cutting of the second phosphodiester bond 3 prime of deoxyinosine and other lesion structures |
| Sample Type | DNA containing deoxyinosine, AP sites or structural lesions |
| Specificity | Recognises deoxyinosine paired or unpaired, AP sites, urea residues, mismatches, hairpins, loops, flaps and Y structures |
| Reaction Conditions / Protocol | Incubate with the DNA substrate under the recommended reaction conditions and resolve the nicked products |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 500 U; 2,500 U |
| Key Features | Broad lesion spectrum beyond deoxyinosine; repair function requires accessory proteins |
| Purity | Greater than 95% |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Nick formation on lesion-containing substrates |
For in vitro diagnostic use.
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