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| Product Name | Endonuclease III (Nth) (10 U/uL) |
| Catalog No. | IVDRM-0570 |
| Description | A bifunctional DNA damage repair enzyme cloned from the nth gene of E. coli, Endonuclease III combines N-glycosylase activity that recognises and excises damaged pyrimidine bases from double stranded DNA to create AP sites, with AP lyase activity that cuts on the 3 prime side of the AP site to give a nick carrying a 5 prime phosphate and a 3 prime open-ring sugar. The enzyme specifically recognises and excises oxidative pyrimidine lesions including urea, 5,6-dihydroxythymine, thymine glycol, 5-hydroxy-5-methylhydantoin, uracil glycol, 6-hydroxy-5,6-dihydrothymine and methyltartronylurea, which supports base excision repair research and in vitro DNA damage repair studies. One unit cuts 1 pmol of a 34 bp oligonucleotide duplex carrying one AP site in one hour at 37°C |
| Intended Use | Base excision repair research on oxidative pyrimidine damage and AP site processing |
| Principle / Technology | Bifunctional N-glycosylase excision of damaged pyrimidines followed by 3 prime side AP lyase cleavage |
| Sample Type | Double stranded DNA with oxidised pyrimidine lesions or AP sites |
| Specificity | Excises urea, dihydroxythymine, thymine glycol, hydroxymethylhydantoin, uracil glycol and related oxidative pyrimidine lesions |
| Reaction Conditions / Protocol | Incubate with the lesion-containing duplex in the dedicated reaction buffer at 37°C |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1000 U |
| Key Features | Dual glycosylase and AP lyase activity with 3 prime side cutting; defined unit on AP site substrate |
| Purity | Greater than or equal to 95% |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Cleavage of AP site containing oligonucleotide duplexes |
For in vitro diagnostic use.
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