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| Product Name | End Repair and A-Tailing Enzyme Mix for NGS Library Preparation |
| Catalog No. | IVDRM-0326 |
| Description | A single-tube enzyme module that completes end repair and A-tailing of fragmented DNA in one reaction. Fragmented input from 500 pg to 1 ug is end polished, phosphorylated at the 5-prime end and extended with a 3-prime A overhang, and the product proceeds straight to adapter ligation without intermediate cleanup, shortening library workflows and protecting low-input material. Formulated for automated handling and dual-platform library construction. |
| Intended Use | End repair and A-tailing of fragmented DNA before adapter ligation in sequencing library construction. |
| Principle / Technology | Combined polymerase and phosphatase activities convert fragmented DNA into ligase-ready molecules with 5-prime phosphates and 3-prime A overhangs. |
| Sample Type | Fragmented DNA (500 pg to 1 ug) |
| Performance Range / Specifications | Handles 500 pg to 1 ug input; single-tube reaction; no intermediate purification required. |
| Specificity | 5-prime phosphorylation and controlled 3-prime A-tailing in one mix. |
| Reaction Conditions / Protocol | Combine fragmented DNA with the mix, incubate, then proceed directly to adapter ligation. |
| Components / Formulation | End repair and A-tailing enzyme mix with reaction buffer. |
| Storage Conditions | -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 24 T, 96 T, 1000 T |
| Product Form | Enzyme and buffer premix |
| Key Features | One-tube workflow; purification-free handoff to ligation; automation-friendly and bulk pack available. |
| Compatibility | Dual-platform sequencing library workflows; automation workstations |
| Starting Material / Tissue Site | 500 pg to 1 ug fragmented DNA |
| Readouts / Downstream Assays | Ligation-ready library DNA |
For in vitro diagnostic use.
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