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| Product Name | EcoRV Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0413 |
| Description | Cutting the palindromic site 5 prime GAT^ATC 3 prime to yield blunt ended fragments, this rapid recombinant endonuclease simplifies downstream ligation design and rapidly processes plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 20 U/uL, it digests in the unified rapid series buffer at 37°C and is heat inactivated at 80°C for 20 minutes. EcoBI methylation possibly overlapping the site may block cutting, and Eco32I is the recognised isoschizomer. This unit stores at -20°C and ships with ice packs. |
| Intended Use | Rapid blunt ended digestion for molecular cloning workflows |
| Principle / Technology | Site specific blunt cleavage at 5 prime GAT^ATC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | EcoBI methylation possibly overlapping the site may block cleavage; isoschizomer Eco32I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 200 T |
| Key Features | Blunt end output; one shared buffer for the whole rapid series |
| Concentration | 20 U/uL |
| Shipping Conditions | Ice packs |
| Readouts / Downstream Assays | Digestion fragment pattern on agarose gel |
For in vitro diagnostic use.
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