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| Product Name | EcoRI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0412 |
| Description | The classic cloning enzyme re-engineered for speed, this rapid recombinant endonuclease cuts 5 prime G^AATTC 3 prime and completes digestion of plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 20 U/uL, it operates in the shared rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. CpG methylation possibly overlapping the sequence obstructs cleavage, and FunII is the documented isoschizomer. Typical uses span DNA fragment cloning and vector construction. This unit stores at -20°C and ships with ice packs. |
| Intended Use | DNA fragment cloning and vector construction with rapid digestion |
| Principle / Technology | Site specific cleavage at 5 prime G^AATTC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation possibly overlapping the site blocks cleavage; isoschizomer FunII |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 600 T |
| Key Features | Large 600 reaction pack for routine cloning; single shared buffer across the series |
| Concentration | 20 U/uL |
| Shipping Conditions | Ice packs |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
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