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| Product Name | EarI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0416 |
| Description | Recognising the asymmetric site 5 prime CTCTTC(N)1^ 3 prime, this rapid recombinant endonuclease cuts outside its recognition sequence, supporting recombinant vector construction, plasmid linearisation and general molecular cloning. It digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes, is supplied at 20 U/uL, and operates in the shared rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes. CpG methylation influences cutting, and Eam1104I and Bst6I are documented isoschizomers. |
| Intended Use | Recombinant vector construction, plasmid linearisation and molecular cloning with distal cleavage |
| Principle / Technology | Site specific cleavage outside 5 prime CTCTTC(N)1 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation affects cleavage; isoschizomers Eam1104I and Bst6I |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 30 T |
| Key Features | Cleavage outside the recognition sequence; one shared buffer across the series |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Digestion profile on agarose gel |
For in vitro diagnostic use.
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