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| Product Name | EagI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0411 |
| Description | Recognising 5 prime C^GGCCG 3 prime, this rapid recombinant endonuclease prepares DNA fragments and cuts genomic DNA for downstream analysis within 5 to 15 minutes. It is supplied at 20 U/uL, digests in the common rapid series buffer at its 37°C optimum, and is heat inactivated at 80°C for 20 minutes. CpG methylation fully overlapping the site blocks cleavage, and the isoschizomers BseX3I, BstZI, EclXI, Eco52I and XmaIII share the same specificity. |
| Intended Use | DNA fragment preparation and genomic DNA analysis with rapid digestion |
| Principle / Technology | Site specific cleavage at 5 prime C^GGCCG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation fully overlapping the site blocks cleavage; isoschizomers BseX3I, BstZI, EclXI, Eco52I and XmaIII |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 25 T |
| Key Features | Single shared buffer across the series; activity redundancy for excess substrate |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Gel profile of digested DNA |
For in vitro diagnostic use.
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