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| Product Name | E. coli Poly(A) Polymerase for RNA 3′ Tailing (5 U/uL) |
| Catalog No. | IVDRM-0366 |
| Description | E. coli poly(A) polymerase, also known as PolyA tailing enzyme, catalyses the template-independent addition of AMP derived from ATP to the 3 end of single-stranded RNA, forming a Poly(A) tail. The reaction uses ATP specifically; dATP is not accepted, CTP and UTP incorporation is below 5% of ATP, and GTP is not incorporated. RNA tailing based on this enzyme has been applied to microbial single-cell sequencing, mRNA in vitro synthesis and miRNA expression analysis. |
| Intended Use | Poly(A) tailing of RNA 3 ends; reverse transcription of tailed RNA; 3 end labelling of RNA; miRNA quantification; mRNA in vitro synthesis; microbial single-cell sequencing |
| Principle / Technology | Template-independent addition of AMP from ATP to the 3 hydroxyl of single-stranded RNA |
| Detection Method | Single-stranded RNA substrates |
| Sample Type | Single-stranded RNA such as miRNA and synthetic transcripts |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 U; 500 U |
| Key Features | High tailing efficiency; ATP-specific; no RNase or exonuclease residue |
| Purity | Free of nuclease, RNase and exonuclease contamination |
| Concentration | 5 U/uL |
For in vitro diagnostic use.
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