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| Product Name | dUTP Solution (100 mM) |
| Catalog No. | IVDRM-0178 |
| Description | dUTP (2'-deoxyuridine-5'-triphosphate) is commonly used in place of dTTP in PCR, qPCR, RT-PCR and RT-qPCR, so that the resulting amplicons contain uracil. When uracil-N-glycosylase (UNG, also known as UDG) is included in the amplification system, uracil residues in any carryover contaminating amplicons are excised, preventing false-positive results. This product is supplied as a ready-to-use sodium salt solution prepared with ultrapure water and adjusted to pH 7.0 with high-purity NaOH, at a concentration of 100 mM. dUTP purity is ≥99% by HPLC, and the solution is tested free of DNase and RNase contamination, so it can be used directly in PCR and other routine molecular biology reactions. English synonym: 2'-Deoxyuridine-5'-triphosphate, sodium salt solution CAS NO.: 102814-08-4 |
| Intended Use | Used as a dTTP substitute in PCR, qPCR, RT-PCR and RT-qPCR to generate uracil-containing amplicons, enabling UDG/UNG-based carryover contamination control to prevent false-positive results. |
| Principle / Technology | Replacing dTTP with dUTP produces uracil-containing amplification products. Uracil-N-glycosylase (UNG/UDG) added to the reaction excises uracil from any contaminating carryover amplicons before amplification, destroying them and thereby eliminating false positives from previous reactions. |
| Performance Range / Specifications | Concentration: 100 mM Purity: ≥99% (HPLC) Appearance: colorless, clear liquid CAS NO.: 102814-08-4 |
| Reaction Conditions / Protocol | Ready to use; add directly to PCR, qPCR, RT-PCR or RT-qPCR setups in place of dTTP. |
| Components / Formulation | dUTP sodium salt solution (100 mM) in ultrapure water, pH adjusted to 7.0 with high-purity NaOH. |
| Storage Conditions | Store at -25°C to -15°C (recommended -20°C). |
| Shelf Life | 2 years when stored at -25°C to -15°C. |
| Package Specifications | 1 mL; 25 mL; 400 μL |
| Product Form | Liquid (colorless, clear, ready-to-use solution) |
| Quality Control | Purity ≥99% verified by HPLC; tested free of DNase and RNase contamination. |
| Purity | ≥99% (HPLC) |
| Concentration | 100 mM |
| pH Range / Optimal pH | pH 7.0 (adjusted with high-purity NaOH) |
| Expiration Date / Stability | Stable for 2 years at -25°C to -15°C. |
| Compatibility | Compatible with PCR, qPCR, RT-PCR and RT-qPCR; intended for use together with uracil-N-glycosylase (UNG/UDG) carryover contamination control systems. |
| Application Notes / Precautions | Storage at -80°C has not been tested; storage at the recommended -20°C is advised. The solution may be diluted with sterile water. The stability of diluted solutions has not been evaluated, so use diluted material as soon as possible. |
For research use only, not for clinical use.
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