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| Product Name | Dulbecco's Phosphate-Buffered Saline Powder, 1×, without Calcium and Magnesium |
| Catalog No. | RERE-0370 |
| Description | Dulbecco's phosphate-buffered saline prepared without calcium and magnesium is also referenced as D-PBSA, CMF-DPBS or simply DPBS, and remains a staple for rinsing cultured cells before dissociation and for countless other everyday culture tasks. The reconstituted 1× solution contains 137 mM sodium chloride, 2.68 mM potassium chloride, 8.1 mM disodium hydrogen phosphate and 1.47 mM potassium dihydrogen phosphate, and settles at pH 7.4 once dissolved. Like other balanced salt solutions, it mirrors the osmotic and acid-base conditions that cells experience during growth and can serve as a diluent for concentrated amino acid or vitamin supplements. |
| Intended Use | Cell washing prior to dissociation, dilution of media supplements and general buffering in cell culture. |
| Principle / Technology | Physiological salt and phosphate chemistry hold osmolality and pH within the range living cells tolerate. |
| Specificity | 1× working concentration after reconstitution; free of calcium and magnesium; pH 7.4 when dissolved. |
| Reaction Conditions / Protocol | Dissolve the powder fully in the stated volume of water. Sterilise by autoclaving or filtration when aseptic handling is needed. |
| Storage Conditions | Room temperature; 24 months. |
| Package Specifications | 1 L, 2 L, 10 L, 10 × 1 L, 10 × 2 L |
| Key Features | D-PBS free of both divalent cations; dissolves to pH 7.4; multiple pack sizes from single litres to bulk packs. |
For research use only, not for clinical use.
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