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| Product Name | Dual-Species Nanobody-Tn5 Fusion Enzyme (anti-mouse and anti-rabbit) for CUT&Tag |
| Catalog No. | IVDRM-0325 |
| Description | A paired set of nanobody-Tn5 fusion enzymes, one recognising mouse primary antibodies and the other rabbit primary antibodies, enabling multi-target profiling at single-cell resolution. When the two enzymes carry adapters with distinct indexes, transposition simultaneously tags the regions bound by mouse-derived and rabbit-derived antibodies, and sequencing reads separate the two targets by index. Supplied as a bare enzyme for user adapter assembly, it supports CUT&Tag, single-cell CUT&Tag and multi-target CUT&Tag designs without secondary antibodies. |
| Intended Use | CUT&Tag, single-cell CUT&Tag and multi-target CUT&Tag library construction; chromatin state and gene regulation studies; neural progenitor and immune cell chromatin profiling. |
| Principle / Technology | Species-specific nanobodies tether Tn5 transposase to primary-antibody-bound chromatin, inserting indexed adapters during tagmentation. |
| Sample Type | Chromatin from cells or single-cell preparations |
| Performance Range / Specifications | Recognises both mouse and rabbit primary antibodies; no secondary antibody needed; index-resolved dual-target readout. |
| Specificity | Anti-mouse and anti-rabbit nanobody specificities in one offering. |
| Reaction Conditions / Protocol | Bind to primary-antibody-labelled chromatin, activate transposition and amplify libraries with index-resolved adapters. |
| Components / Formulation | Anti-mouse nanobody-Tn5 and anti-rabbit nanobody-Tn5 fusion enzymes. |
| Storage Conditions | -85 to -65 °C |
| Shelf Life | 1 year |
| Package Specifications | 10 uL, 80 uL, 100 uL |
| Product Form | Bare enzyme solution |
| Key Features | Two species in one product; single-cell multi-target capability; adapter-flexible bare format. |
| Source / Origin | Recombinant nanobody-Tn5 fusion proteins |
| Readouts / Downstream Assays | CUT&Tag and scCUT&Tag chromatin libraries |
For in vitro diagnostic use.
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