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| Product Name | DraI Rapid Restriction Endonuclease (5 U/uL) |
| Catalog No. | IVDRM-0407 |
| Description | Cutting the AT rich blunt site 5 prime TTT^AAA 3 prime, this rapid recombinant endonuclease supports molecular cloning, vector construction and plasmid linearisation with clean digestion of plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. It is supplied at 5 U/uL and performs in the common rapid series buffer at 37°C, with heat inactivation at 80°C for 20 minutes. EcoKI methylation fully overlapping the site blocks cleavage while no alternative named isoschizomers are documented. The AT rich recognition sequence is relatively rare in GC rich genomes, giving the enzyme niche value for selective linearisation. |
| Intended Use | Molecular cloning, vector construction and plasmid linearisation with rapid digestion |
| Principle / Technology | Site specific blunt cleavage at 5 prime TTT^AAA 3 prime within 5 to 15 minutes at 37°C |
| Specificity | EcoKI methylation fully overlapping the site blocks cleavage; no alternative named isoschizomers |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | AT rich rare cutter; single shared buffer across the rapid series |
| Concentration | 5 U/uL |
| Readouts / Downstream Assays | Digestion profile on agarose gel |
For in vitro diagnostic use.
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